TRACHTOVÁ, Štěpánka, Ondřel KAMAN, Alena ŠPANOVÁ, Pavel VEVERKA, Emil POLLERT and Bohuslav RITTICH. Silica coated La0.75Sr0.25MnO3 nanoparticles for magnetically driven DNA isolation. (Silica coated La0.75Sr0.25MnO3 nanoparticles for magnetically driven DNA isolation.). Journal of Separation Science. Weinheim: Wiley-VCH, 2011, vol. 34, No 21, p. 3077-3082. ISSN 1615-9306. Available from: https://dx.doi.org/10.1002/jssc.201100442.
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Basic information
Original name Silica coated La0.75Sr0.25MnO3 nanoparticles for magnetically driven DNA isolation.
Name in Czech Nanočástice La0.75Sr0.25MnO3 pokryté silikagelem pro magneticky řízenou izolaci DNA
Authors TRACHTOVÁ, Štěpánka (203 Czech Republic), Ondřel KAMAN (203 Czech Republic), Alena ŠPANOVÁ (203 Czech Republic, belonging to the institution), Pavel VEVERKA (203 Czech Republic), Emil POLLERT (203 Czech Republic) and Bohuslav RITTICH (203 Czech Republic, guarantor, belonging to the institution).
Edition Journal of Separation Science, Weinheim, Wiley-VCH, 2011, 1615-9306.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10600 1.6 Biological sciences
Country of publisher Germany
Confidentiality degree is not subject to a state or trade secret
Impact factor Impact factor: 2.733
RIV identification code RIV/00216224:14310/11:00053297
Organization unit Faculty of Science
Doi http://dx.doi.org/10.1002/jssc.201100442
UT WoS 000297415600020
Keywords (in Czech) izolace DNA; lanthanito manganatý perovskit; nanočástice; PCR; silikagelové pokrytí
Keywords in English DNA isolation; lanthanum manganese perovskite; nanoparticles; PCR; silica shell
Tags AKR, rivok, ZR
Tags International impact, Reviewed
Changed by Changed by: Ing. Andrea Mikešková, učo 137293. Changed: 31/7/2013 10:59.
Abstract
Magnetic La0.75Sr0.25MnO3 nanoparticles possessing an approximately 20 nm thick silica shell (LSMO(0.25)@SiO2) were characterised and tested for the isolation of PCR-ready bacterial DNA. The results presented here show that thenanoparticles do not interfere in PCR. DNA was reversibly adsorbed on their silica shell from the aqueous phase system (16 % PEG 6000 – 2 M NaCl). The method proposed was used for DNA isolation from complex food samples (dairy products and probiotic food supplements). The isolated DNA was compatible with PCR. The main advantages of the nanoparticles tested for routine use were their high colloidal stability allowing a more precise dosage and therefore high reproducibility of DNA isolation.
Abstract (in Czech)
Magnetické La0.75Sr0.25MnO3 nanočástice, pokryté asi 20 nm silnou vrstvou silikagelu LSMO(0.25)@SiO2), byly testovány pro izolaci DNA v kvalitě vhodné pro PCR. Bylo zjištěno, že nanočástice neinterferují v PCR. DNA byla reversibilně adsorbována na siligalový povrch částic z vodného systému (16 % PEG 6000 – 2 M NaCl). VyvinutáNavržená metoda byla použita k izolaci DNA komplexních vzorků potravin (mléčné výrobky a probiotické potravinové doplňky). Izolovaná DNA byla amplifikovatelná v PCR. Hlavní výhodou nanočástic při běžném použití byla jejich vysoká koloidní stabilita, která umožňovala přesnější dávkování a tudíž vysokou reprodukovatelnost izolace DNA.
Links
MSM0021622415, plan (intention)Name: Molekulární podstata buněčných a tkáňových regulací
Investor: Ministry of Education, Youth and Sports of the CR, Molecular basis of cell and tissue regulations
2B06053, research and development projectName: Nové postupy při charakterizaci a identifikaci probiotických kmenů bakterií vhodných pro funkční potraviny.
Investor: Ministry of Education, Youth and Sports of the CR, New methods for the characterization and identification of probiotic bacterial strains suitable for functional foods
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