The Effect of St. Johns Wort (Hypericum Perforatum) on
Cytochrome  P450 1A2 Activity in Perfused Rat Liver

J 2011

The Effect of St. Johns Wort (Hypericum Perforatum) on Cytochrome P450 1A2 Activity in Perfused Rat Liver

DOSTÁLEK, Miroslav, Jana PISTOVČÁKOVÁ, Jan JUŘICA, Alexandra ŠULCOVÁ, Josef TOMANDL et. al.

Basic information

Original name

The Effect of St. Johns Wort (Hypericum Perforatum) on Cytochrome P450 1A2 Activity in Perfused Rat Liver

Authors

DOSTÁLEK, Miroslav (203 Czech Republic, belonging to the institution), Jana PISTOVČÁKOVÁ (203 Czech Republic, belonging to the institution), Jan JUŘICA (203 Czech Republic, belonging to the institution), Alexandra ŠULCOVÁ (203 Czech Republic, belonging to the institution) and Josef TOMANDL (203 Czech Republic, guarantor, belonging to the institution)

Edition

Biomedical papers of the Medical Faculty of the University Palacký, Olomouc Czech Republic, 2011, 1213-8118

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30104 Pharmacology and pharmacy

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Biomed Pap 2011;155(3):253–258

Impact factor

Impact factor: 0.702

RIV identification code

RIV/00216224:14740/11:00050036

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.5507/bp.2011.047

UT WoS

000297807500005

Keywords in English

St John‘s wort; Hypericum perforatum; Cytochrome P450; Liver isolated perfused; Rat

Abstract

V originále

We previously reported the effect of St John’s wort (Hypericum perforatum) standardised extract LI 160 on the activities of cytochrome P450 2C6, 2D2 and 3As (Dostalek et al., Life Sciences 2005;78(3):239-44). In this study, we aimed to assess the effect of St John’s wort on the activity of cytochrome P450 1A2. The isolated perfused rat liver model was used in our experiments with phenacetin as a marker substrate for cytochrome P450 1A2. The rate of formation of acetaminophen was significantly inhibited by the use of St John’s wort. In addition, St John‘s wort extract inhibited cytochrome P450 1A2 activity significantly more than the control inhibitor. St John’s wort significantly inhibited cytochrome P450 1A2 enzyme activity. It remains to be determined whether the co-administration of St John‘s wort extract and other medications or substrates for cytochrome P450 1A2 could result in significant drug interactions.

Links

GAP206/10/0057, research and development project

Name: Miniaturizovaný systém pro "on-line" studie metabolismu léčiv založený na kapilární elektroforéze

Investor: Czech Science Foundation, Miniaturized on-line drug metabolism system based on capillary electrophoresis

LC06023, research and development project

Name: Integrované bioanalytické technologie pro mikroanalýzy a diagnostiku s využitím LIF a hmotnostní spektrometrie

Investor: Ministry of Education, Youth and Sports of the CR

MSM0021622404, plan (intention)

Name: Vnitřní organizace a neurobiologické mechanismy funkčních systémů CNS

Investor: Ministry of Education, Youth and Sports of the CR, The internal organisation and neurobiological mechanisms of functional CNS systems under normal and pathological conditions.

Citovat

DOSTÁLEK, Miroslav, Jana PISTOVČÁKOVÁ, Jan JUŘICA, Alexandra ŠULCOVÁ and Josef TOMANDL. The Effect of St. Johns Wort (Hypericum Perforatum) on Cytochrome P450 1A2 Activity in Perfused Rat Liver. Biomedical papers of the Medical Faculty of the University Palacký, Olomouc Czech Republic. 2011, vol. 155, No 3, p. 253-257. ISSN 1213-8118. Available from: https://dx.doi.org/10.5507/bp.2011.047.

@article{952508,
   author = {Dostálek, Miroslav and Pistovčáková, Jana and Juřica, Jan and Šulcová, Alexandra and Tomandl, Josef},
   article_number = {3},
   doi = {http://dx.doi.org/10.5507/bp.2011.047},
   keywords = {St John‘s wort; Hypericum perforatum; Cytochrome P450; Liver isolated perfused; Rat},
   language = {eng},
   issn = {1213-8118},
   journal = {Biomedical papers of the Medical Faculty of the University Palacký, Olomouc Czech Republic},
   title = {The Effect of St. Johns Wort (Hypericum Perforatum) on Cytochrome P450 1A2 Activity in Perfused Rat Liver},
   url = {http://biomed.papers.upol.cz/pdfs/bio/2011/03/05.pdf},
   volume = {155},
   year = {2011}
}

TY  - JOUR
ID  - 952508
AU  - Dostálek, Miroslav - Pistovčáková, Jana - Juřica, Jan - Šulcová, Alexandra - Tomandl, Josef
PY  - 2011
TI  - The Effect of St. Johns Wort (Hypericum Perforatum) on Cytochrome P450 1A2 Activity in Perfused Rat Liver
JF  - Biomedical papers of the Medical Faculty of the University Palacký, Olomouc Czech Republic
VL  - 155
IS  - 3
SP  - 253-257
EP  - 253-257
SN  - 12138118
KW  - St John‘s wort
KW  - Hypericum perforatum
KW  - Cytochrome P450
KW  - Liver isolated perfused
KW  - Rat
UR  - http://biomed.papers.upol.cz/pdfs/bio/2011/03/05.pdf
L2  - http://biomed.papers.upol.cz/pdfs/bio/2011/03/05.pdf
N2  - We previously reported the effect of St John’s wort (Hypericum perforatum) standardised extract LI 160 on the activities of cytochrome P450 2C6, 2D2 and 3As (Dostalek et al., Life Sciences 2005;78(3):239-44). In this study, we aimed to assess the effect of St John’s wort on the activity of cytochrome P450 1A2. The isolated perfused rat liver model was used in our experiments with phenacetin as a marker substrate for cytochrome P450 1A2. The rate of formation of acetaminophen was significantly inhibited by the use of St John’s wort. In addition, St John‘s wort extract inhibited cytochrome P450 1A2 activity significantly more than the control inhibitor. St John’s wort significantly inhibited cytochrome P450 1A2 enzyme activity. It remains to be determined whether the co-administration of St John‘s wort extract and other medications or substrates for cytochrome P450 1A2 could result in significant drug interactions.
ER  -

DOSTÁLEK, Miroslav, Jana PISTOVČÁKOVÁ, Jan JUŘICA, Alexandra ŠULCOVÁ and Josef TOMANDL. The Effect of St. Johns Wort (Hypericum Perforatum) on Cytochrome P450 1A2 Activity in Perfused Rat Liver. \textit{Biomedical papers of the Medical Faculty of the University Palacký, Olomouc Czech Republic}. 2011, vol.~155, No~3, p.~253-257. ISSN~1213-8118. Available from: https://dx.doi.org/10.5507/bp.2011.047.

Detailed Information on Publication Record