The effects of PAHs and N-PAHs on retinoid signalling and Oct-4 expression in vitro.

BENÍŠEK, Martin, Petra KUBINCOVÁ, Luděk BLÁHA and Klára HILSCHEROVÁ. The effects of PAHs and N-PAHs on retinoid signalling and Oct-4 expression in vitro. Toxicology Letters. Clare (Ireland): Elsevier Ireland Ltd, 2011, vol. 200, No 3, p. 169-175. ISSN 0378-4274. Available from: https://dx.doi.org/10.1016/j.toxlet.2010.11.011.

Basic information

• Original name: The effects of PAHs and N-PAHs on retinoid signalling and Oct-4 expression in vitro.
• Name in Czech: Efekty PAH a N-PAH na signálování retinoidů a expresi Oct-4 in vitro
• Authors: BENÍŠEK, Martin (203 Czech Republic, belonging to the institution), Petra KUBINCOVÁ (203 Czech Republic, belonging to the institution), Luděk BLÁHA (203 Czech Republic, guarantor, belonging to the institution) and Klára HILSCHEROVÁ (203 Czech Republic, belonging to the institution).
• Edition: Toxicology Letters, Clare (Ireland), Elsevier Ireland Ltd, 2011, 0378-4274.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: 30304 Public and environmental health
• Country of publisher: Ireland
• Confidentiality degree: is not subject to a state or trade secret
• Impact factor: Impact factor: 3.230
• RIV identification code: RIV/00216224:14310/11:00053414
• Organization unit: Faculty of Science
• Doi: http://dx.doi.org/10.1016/j.toxlet.2010.11.011
• UT WoS: 000287216900006
• Keywords in English: retinoids; differentiation; Oct-4; PAHs; N-PAHs
• Tags: AKR, rivok
• Tags: International impact, Reviewed

Abstract

This study investigated PAHs and N-PAHs interference with retinoid signaling in vitro by modulating all-trans retinoic acid (ATRA) mediated response in a reporter gene assay using P19/A15 cell line. Further, effects on pluripotency and differentiation processes were evaluated by measuring octamer-4 (Oct-4), an important pluripotency marker and master differentiation factor. Our results further extend the knowledge of PAH and N-PAH in vitro effects and indicate that these environmental toxicants may have influence on differentiation process and embryonic development by interfering with ATRA signaling and by modulating levels of Oct-4.

Abstract (in Czech)

Studie zkoumá interferenci PAH a N-PAH s kyselinou retinovou (ATRA) na signálování retinoidů s využitím buněčných linií P19/A15 v gene report assay. Dále byly zkoumány efekty na pluripotentnost a diferenciační proces měřením Oct-4. Výsledek in vitro experimentů ukázal možný vliv vybraných toxikantů na diferenciační proces a vývoj embryí.

Links

• ED0001/01/01, research and development project: Name: CETOCOEN
• MSM0021622412, plan (intention): Name: Interakce mezi chemickými látkami, prostředím a biologickými systémy a jejich důsledky na globální, regionální a lokální úrovni (INCHEMBIOL) (Acronym: INCHEMBIOL)

Investor: Ministry of Education, Youth and Sports of the CR, Interactions among the chemicals, environment and biological systems and their consequences on the global, regional and local scales (INCHEMBIOL)

• 2B08036, research and development project: Name: Nové molekulárně biologické a biochemické metody pro monitoring estrogenů a dalších chemických endokrinních disruptorů (Acronym: ENVISCREEN)

Investor: Ministry of Education, Youth and Sports of the CR, New molecular biology and biochemical methods to monitor estrogens and other endocrine disruptors in the environment of the C.R.