VARGA, Marian, Lucie KUNTOVÁ, Ivana MAŠLAŇOVÁ, Vladislava RŮŽIČKOVÁ, Roman PANTŮČEK and Jiří DOŠKAŘ. Transduction of penicilinase plasmids within community-acquired methicillin-resistant Staphylococcus aureus USA 300 clone. In 4th Congress of European Microbiologists FEMS 2011, Geneva, Switzerland. 2011.
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Basic information
Original name Transduction of penicilinase plasmids within community-acquired methicillin-resistant Staphylococcus aureus USA 300 clone
Authors VARGA, Marian, Lucie KUNTOVÁ, Ivana MAŠLAŇOVÁ, Vladislava RŮŽIČKOVÁ, Roman PANTŮČEK and Jiří DOŠKAŘ.
Edition 4th Congress of European Microbiologists FEMS 2011, Geneva, Switzerland, 2011.
Other information
Original language English
Type of outcome Conference abstract
Field of Study Genetics and molecular biology
Country of publisher Switzerland
Confidentiality degree is not subject to a state or trade secret
WWW URL
Organization unit Faculty of Science
Tags International impact, Reviewed
Changed by Changed by: prof. RNDr. Roman Pantůček, Ph.D., učo 842. Changed: 14/10/2011 14:04.
Abstract
Two penicillinase plasmids from four different donor strains were transduced to a recipient strain. High frequencies of transduction were observer both in propagated as well as induced phage lysates. The presence of plasmids in phage particles and the frequency of their transfer by transduction were studied by real-time PCR. These results have shown efficient transfer of resistance plasmids between strains of USA300 clone. Moreover, recently isolated phage phiJB proved outstanding transduction ability and has been chosen for further molecular analysis including whole-genome sequencing.
Links
GA310/09/0459, research and development projectName: Úloha bakteriofágů v horizontálním přenosu genů virulence a rezistence u Staphylococcus aureus
Investor: Czech Science Foundation, Role of bacteriophages in horizontal transfer of virulence and drug resistance genes in Staphylococcus aureus
MSM0021622415, plan (intention)Name: Molekulární podstata buněčných a tkáňových regulací
Investor: Ministry of Education, Youth and Sports of the CR, Molecular basis of cell and tissue regulations
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