a 2011

Telomere maintenance in plant cells with hypomethylated telomeres and subtelomeres

MAJEROVÁ, Eva, Miloslava FOJTOVÁ, Iva MOZGOVÁ and Jiří FAJKUS

Basic information

Original name

Telomere maintenance in plant cells with hypomethylated telomeres and subtelomeres

Name in Czech

Udržování stabilních telomer v rostlinných buňkách s hypomethylovanými telomerami a subtelomerami

Authors

MAJEROVÁ, Eva, Miloslava FOJTOVÁ, Iva MOZGOVÁ and Jiří FAJKUS

Edition

TELOMERES and TELOMERASE, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, 2011

Other information

Language

English

Type of outcome

Konferenční abstrakt

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

Organization unit

Faculty of Science
Změněno: 14/10/2011 15:30, Mgr. Eva Majerová, Ph.D.

Abstract

V originále

Telomeres, regarding to the recent results, display euchromatic and heterochromatic features and their maintenance is co-regulated epigenetically. Mice deficient for histone or DNA methyltransferases showed, among others, increased length of telomeres and noticeable changes in their chromatin structure. Here we studied the effect of hypomethylating drugs on telomere maintenance in plants, which show much higher complexity of epigenetic pathways compared to mammals. We reached a considerable hypomethylation of whole genome and even deeper hypomethylation of subtelomeric and telomeric regions (up to six times bigger than in control cells); when the reagents are eliminated the re-methylation occurs immediately. As regards to nucleosomal positioning, no visible changes were detected but considerable changes were seen in the level of TERRA. For cells treated by DHPA the level of TERRA rapidly decreased while cells treated by zebularine showed a remarkable increase in TERRA. This divergence can be caused by a different sphere of action of both reagents. The qTRAP assay revealed that in treated cells the activity of telomerase significantly increases but returns to the control level as soon as the hypomethylating reagents are removed which is in an exact accord to the changes in methylation pattern. Surprisingly when we measured the length of telomeres in control and treated cells we didn’t observe any changes which indicates that the length of telomeres is not directly bounded to the activity of telomerase and the increased activity of telomerase doesn’t necessarily lead to the elongation of telomeres in tobacco plants.

Links

AV0Z50040507, plan (intention)
Name: Biofyzika dynamických struktur a funkcí biologických systémů
GA204/08/1530, research and development project
Name: Objasnění úlohy proteinu HMGB1 v udržování genomové stability
Investor: Czech Science Foundation, Understanding of HMGB1 protein involvement in maintaining genome stability
LC06004, research and development project
Name: Integrovaný výzkum rostlinného genomu
MSM0021622415, plan (intention)
Name: Molekulární podstata buněčných a tkáňových regulací
Investor: Ministry of Education, Youth and Sports of the CR, Molecular basis of cell and tissue regulations
MUNI/G/0104/2009, interní kód MU
Name: Vliv struktury chromatinu na regulaci telomer
Investor: Masaryk University, Category G - development project + specific research