A new approach how to define coefficient of electroactivity of adenine and its twelve derivatives using flow injection analysis with amperometric detection.

ZÍTKA, Ondřej, Helena SKUTKOVA, Vojtěch ADAM, Libuše TRNKOVÁ, Petr BABULA, Jaromír HUBÁLEK, Ivo PROVAZNÍK and René KIZEK. A new approach how to define coefficient of electroactivity of adenine and its twelve derivatives using flow injection analysis with amperometric detection. Electroanalysis. 2011, vol. 23, No 7, p. 1556-1567. ISSN 1040-0397. Available from: https://dx.doi.org/10.1002/elan.201100064.

Basic information

• Original name: A new approach how to define coefficient of electroactivity of adenine and its twelve derivatives using flow injection analysis with amperometric detection.
• Authors: ZÍTKA, Ondřej (203 Czech Republic), Helena SKUTKOVA (203 Czech Republic), Vojtěch ADAM (203 Czech Republic), Libuše TRNKOVÁ (203 Czech Republic, belonging to the institution), Petr BABULA (203 Czech Republic), Jaromír HUBÁLEK (203 Czech Republic), Ivo PROVAZNÍK (203 Czech Republic) and René KIZEK (203 Czech Republic, guarantor).
• Edition: Electroanalysis, 2011, 1040-0397.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: 10403 Physical chemistry
• Country of publisher: Czech Republic
• Confidentiality degree: is not subject to a state or trade secret
• WWW: URL
• Impact factor: Impact factor: 2.872
• RIV identification code: RIV/00216224:14310/11:00053556
• Organization unit: Faculty of Science
• Doi: http://dx.doi.org/10.1002/elan.201100064
• UT WoS: 000292967900007
• Keywords in English: Adenine derivatives; Flow injection analysis; Amperometric detection; Glassy carbon electrode; Electroactivity
• Tags: AKR, rivok

Abstract

We studied the electrochemical behaviour of adenine derivates (adenosine, 2-aminopurine, 2,6-diaminopurine, 6-benzyl-aminopurine, adenosine monophosphate, cyclic adenosine monophosphate, nicotinamide adenine dinucleotide, adenosine triphosphate, S-adenosyl-L-methionine, and synthetic derivatives AD-3, AD-6 and AD-9) using flow injection analysis with electrochemical detection using a glassy carbon electrode. The influences of pH, flow rate and potential on the signal height of the studied derivates were tested. The optimal pH was 3, the flow rate of the mobile phase 0.75 mLmin(-1) and the potential 1100 mV. Further, we attempted to characterize each of the studied derivatives by mathematical equations and classic analytical parameters. The lowest detection limit was estimated for adenine as 0.9 nM and 2-aminopurine as 0.5 nM.

Links

• MSM0021622412, plan (intention): Name: Interakce mezi chemickými látkami, prostředím a biologickými systémy a jejich důsledky na globální, regionální a lokální úrovni (INCHEMBIOL) (Acronym: INCHEMBIOL)

Investor: Ministry of Education, Youth and Sports of the CR, Interactions among the chemicals, environment and biological systems and their consequences on the global, regional and local scales (INCHEMBIOL)