A solution study of protonation chemical equlibria of nucleobases and short oligonucleotides

LUBAL, Přemysl, Sylvie HOLUBOVÁ and Libuše TRNKOVÁ. A solution study of protonation chemical equlibria of nucleobases and short oligonucleotides. In Biopolymers: Structure and dynamics, A biophysical workshop. ISBN 978-80-263-0050-2. 2011.

Basic information

• Original name: A solution study of protonation chemical equlibria of nucleobases and short oligonucleotides
• Authors: LUBAL, Přemysl (203 Czech Republic, guarantor, belonging to the institution), Sylvie HOLUBOVÁ (203 Czech Republic, belonging to the institution) and Libuše TRNKOVÁ (203 Czech Republic, belonging to the institution).
• Edition: Biopolymers: Structure and dynamics, A biophysical workshop, 2011.

Other information

• Original language: English
• Type of outcome: Conference abstract
• Field of Study: 10406 Analytical chemistry
• Country of publisher: Czech Republic
• Confidentiality degree: is not subject to a state or trade secret
• RIV identification code: RIV/00216224:14310/11:00053670
• Organization unit: Faculty of Science
• ISBN: 978-80-263-0050-2
• Keywords in English: oligonucleotides;cytosine;adenine;molecular spectroscopy;equispec;opium
• Tags: AKR, ZR

Abstract

The protonation constants of nucleobases and relevant nucleosides and nucleotides were determined by means of potenciometry and molecular absorption spectroscopy in UV region (I=0.15 mol.l-1 NaCl,t=25C) and the experimental data were evaluated by OPIUM program. In addition, thermodynamic parameters for nucleobase ligands were estimated. Also, some examples of ligand complexation by transition metal ions (e.g. copper(II),zinc(II),kadmium(II)) will be presented and their importance for bioligand speciation will be discussed. The protonation behavior of ODN containing C, i.e.(CCC)n,n=1–3, was followed by means of molecular spectroscopy in pH region 2–8 and variable temperature and ionic strength adjusted by NaCl. The experimental data were treated by means of OPIUM, MCR – ALS and EQUISPEC software and distribution diagram of species as pH fuction were calculated. For comparison, the protonation behavior of ODN with the (CAC)3, A3C3C3 and C3A3C3 sequence was also investigated. The influence of the structural motif and length on protonation constants o studied ODN seems to be very important due to probace stabilization of the secondary structure of the biomacromolecule.

Links

• LC06035, research and development project: Name: Centrum biofyzikální chemie, bioelektrochemie a bioanalýzy. Nové nástroje pro genomiku, proteomiku a biomedicínu.

Investor: Ministry of Education, Youth and Sports of the CR, Centre of Biophysical Chemistry, Bioelectrochemistry and Bioanalysis. New Tools for Genomics, Proteomics and Biomedicine

• ME09065, research and development project: Name: Výzkum nových detekčních systémů na bázi senzorových polí pro použití ve speciační analýze

Investor: Ministry of Education, Youth and Sports of the CR, Research of new detection systems based on sensor arrays for application in speciation analysis, Research and Development Programme KONTAKT (ME)

• MUNI/A/0992/2009, interní kód MU: Name: Vývoj metodologie a instrumentace analytické chemie pro studium složení a vlastností přírodních a syntetických materiálů (Acronym: MIA)

Investor: Masaryk University, Category A