A solution study of protonation chemical equlibria of nucleobases and short oligonucleotides

LUBAL, Přemysl, Sylvie HOLUBOVÁ and Libuše TRNKOVÁ. A solution study of protonation chemical equlibria of nucleobases and short oligonucleotides. In Biopolymers: Structure and dynamics, A biophysical workshop. 2011. ISBN 978-80-263-0050-2.

Basic information

Original name

A solution study of protonation chemical equlibria of nucleobases and short oligonucleotides

Authors

LUBAL, Přemysl (203 Czech Republic, guarantor, belonging to the institution), Sylvie HOLUBOVÁ (203 Czech Republic, belonging to the institution) and Libuše TRNKOVÁ (203 Czech Republic, belonging to the institution)

Edition

Biopolymers: Structure and dynamics, A biophysical workshop, 2011

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Other information

Language

English

Type of outcome

Konferenční abstrakt

Field of Study

10406 Analytical chemistry

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14310/11:00053670

Organization unit

Faculty of Science

ISBN

978-80-263-0050-2

Keywords in English

oligonucleotides;cytosine;adenine;molecular spectroscopy;equispec;opium

Tags

AKR, ZR

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Abstract

V originále

The protonation constants of nucleobases and relevant nucleosides and nucleotides were determined by means of potenciometry and molecular absorption spectroscopy in UV region (I=0.15 mol.l-1 NaCl,t=25C) and the experimental data were evaluated by OPIUM program. In addition, thermodynamic parameters for nucleobase ligands were estimated. Also, some examples of ligand complexation by transition metal ions (e.g. copper(II),zinc(II),kadmium(II)) will be presented and their importance for bioligand speciation will be discussed. The protonation behavior of ODN containing C, i.e.(CCC)n,n=1–3, was followed by means of molecular spectroscopy in pH region 2–8 and variable temperature and ionic strength adjusted by NaCl. The experimental data were treated by means of OPIUM, MCR – ALS and EQUISPEC software and distribution diagram of species as pH fuction were calculated. For comparison, the protonation behavior of ODN with the (CAC)3, A3C3C3 and C3A3C3 sequence was also investigated. The influence of the structural motif and length on protonation constants o studied ODN seems to be very important due to probace stabilization of the secondary structure of the biomacromolecule.

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Links

LC06035, research and development project	Name: Centrum biofyzikální chemie, bioelektrochemie a bioanalýzy. Nové nástroje pro genomiku, proteomiku a biomedicínu. Investor: Ministry of Education, Youth and Sports of the CR, Centre of Biophysical Chemistry, Bioelectrochemistry and Bioanalysis. New Tools for Genomics, Proteomics and Biomedicine
ME09065, research and development project	Name: Výzkum nových detekčních systémů na bázi senzorových polí pro použití ve speciační analýze Investor: Ministry of Education, Youth and Sports of the CR, Research of new detection systems based on sensor arrays for application in speciation analysis, Research and Development Programme KONTAKT (ME)
MUNI/A/0992/2009, interní kód MU	Name: Vývoj metodologie a instrumentace analytické chemie pro studium složení a vlastností přírodních a syntetických materiálů (Acronym: MIA) Investor: Masaryk University, Category A