J 2011

Structure and binding specificity of the receiver domain of sensor histidine kinase CKI1 from Arabidopsis thaliana.

PEKÁROVÁ, Blanka, Tomáš KLUMPLER, Olga TŘÍSKOVÁ, Jakub HORÁK, Séverine JANSEN et. al.

Basic information

Original name

Structure and binding specificity of the receiver domain of sensor histidine kinase CKI1 from Arabidopsis thaliana.

Authors

PEKÁROVÁ, Blanka (203 Czech Republic, belonging to the institution), Tomáš KLUMPLER (203 Czech Republic, belonging to the institution), Olga TŘÍSKOVÁ (203 Czech Republic, belonging to the institution), Jakub HORÁK (203 Czech Republic, belonging to the institution), Séverine JANSEN (250 France, belonging to the institution), Radka DOPITOVÁ (203 Czech Republic, belonging to the institution), Petra BORKOVCOVÁ (203 Czech Republic, belonging to the institution), Veronika PAPOUŠKOVÁ (203 Czech Republic, belonging to the institution), Eliška NEJEDLÁ (203 Czech Republic, belonging to the institution), Vladimír SKLENÁŘ (203 Czech Republic, belonging to the institution), Jaromír MAREK (203 Czech Republic, belonging to the institution), Lukáš ŽÍDEK (203 Czech Republic, belonging to the institution), Jan HEJÁTKO (203 Czech Republic, belonging to the institution) and Lubomír JANDA (203 Czech Republic, guarantor, belonging to the institution)

Edition

The Plant Journal, England, BLACKWELL PUBLISHING, 2011, 0960-7412

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

Genetics and molecular biology

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Impact factor

Impact factor: 6.160

RIV identification code

RIV/00216224:14740/11:00050106

Organization unit

Central European Institute of Technology

DOI

UT WoS

000294827700008

Keywords in English

multistep phosphorelay; receiver domain; CKI1; crystal structure; NMR spectroscopy; Arabidopsis thaliana

Tags

Tags

International impact, Reviewed
Změněno: 1/5/2012 01:49, doc. RNDr. Jaromír Marek, Ph.D.

Abstract

V originále

Multistep phosphorelay (MSP) signaling mediates responses to a variety of important stimuli in plants. In Arabidopsis MSP, the signal is transferred from sensor histidine kinase (HK) via histidine phosphotransfer proteins (AHP1–AHP5) to nuclear response regulators. In contrast to ancestral two-component signaling in bacteria, protein interactions in plant MSP are supposed to be rather nonspecific. Here, we show that the C-terminal receiver domain of HK CKI1 (CKI1RD) is responsible for the recognition of CKI1 downstream signaling partners, and specifically interacts with AHP2, AHP3 and AHP5 with different affinities. We studied the effects of Mg2+, the co-factor necessary for signal transduction via MSP, and phosphorylation-mimicking BeF3 ) on CKI1RD in solution, and determined the crystal structure of free CKI1RD and CKI1RD in a complex with Mg2+. We found that the structure of CKI1RD shares similarities with the only known structure of plant HK, ETR1RD, with the main differences being in loop L3.

Links

ED1.1.00/02.0068, research and development project
Name: CEITEC - central european institute of technology
GAP305/11/0756, research and development project
Name: Strukturní princip specificity přenosu signálu v rostlinách: síť interakcí příjímačové domény histidinkinas u Arabidospis (Acronym: HKRD-AHP)
Investor: Czech Science Foundation
GA521/09/1699, research and development project
Name: Imunomodulace jako nástroj funkční proteomiky při studiu cytokininové signální dráhy u Arabidopsis thaliana
Investor: Czech Science Foundation, Immunomodulation as a functional proteomics tool for cytokinin signaling study in Arabidopsis thaliana
GD204/08/H054, research and development project
Name: Molekulární mechanismy proliferace a diferenciace buněk
Investor: Czech Science Foundation, Molecular mechanisms of the cell proliferation and differentiation
LC06034, research and development project
Name: Regulace morfogeneze rostlinných buněk a orgánů
Investor: Ministry of Education, Youth and Sports of the CR, Regulation of morphogenesis of plant cells and organs
MSM0021622413, plan (intention)
Name: Proteiny v metabolismu a při interakci organismů s prostředím
Investor: Ministry of Education, Youth and Sports of the CR, Proteins in metabolism and interaction of organisms with the environment
MSM0021622415, plan (intention)
Name: Molekulární podstata buněčných a tkáňových regulací
Investor: Ministry of Education, Youth and Sports of the CR, Molecular basis of cell and tissue regulations
MUNI/A/0928/2009, interní kód MU
Name: Struktura a dynamika biopolymerů (Acronym: SDB)
Investor: Masaryk University, Category A
205872, interní kód MU
Name: Program developing interdisciplinary research POtential for the STudies of BIOMolecular INteractions (Acronym: POSTBIOMIN)
Investor: European Union, Program developing interdisciplinary research POtential for the STudies of BIOMolecular INteractions, Capacities
228461, interní kód MU
Name: Enhancing Access and Services to East European users Towards an efficient (Acronym: EAST-NMR)
Investor: European Union, Enhancing Access and Services to East European users Towards an efficient, Capacities