PEKÁROVÁ, Blanka, Tomáš KLUMPLER, Olga TŘÍSKOVÁ, Jakub HORÁK, Séverine JANSEN, Radka DOPITOVÁ, Petra BORKOVCOVÁ, Veronika PAPOUŠKOVÁ, Eliška NEJEDLÁ, Vladimír SKLENÁŘ, Jaromír MAREK, Lukáš ŽÍDEK, Jan HEJÁTKO and Lubomír JANDA. Structure and binding specificity of the receiver domain of sensor histidine kinase CKI1 from Arabidopsis thaliana. The Plant Journal. England: BLACKWELL PUBLISHING, 2011, vol. 67, No 5, p. 827-839. ISSN 0960-7412. Available from: https://dx.doi.org/10.1111/j.1365-313X.2011.04637.x.
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Basic information
Original name Structure and binding specificity of the receiver domain of sensor histidine kinase CKI1 from Arabidopsis thaliana.
Authors PEKÁROVÁ, Blanka (203 Czech Republic, belonging to the institution), Tomáš KLUMPLER (203 Czech Republic, belonging to the institution), Olga TŘÍSKOVÁ (203 Czech Republic, belonging to the institution), Jakub HORÁK (203 Czech Republic, belonging to the institution), Séverine JANSEN (250 France, belonging to the institution), Radka DOPITOVÁ (203 Czech Republic, belonging to the institution), Petra BORKOVCOVÁ (203 Czech Republic, belonging to the institution), Veronika PAPOUŠKOVÁ (203 Czech Republic, belonging to the institution), Eliška NEJEDLÁ (203 Czech Republic, belonging to the institution), Vladimír SKLENÁŘ (203 Czech Republic, belonging to the institution), Jaromír MAREK (203 Czech Republic, belonging to the institution), Lukáš ŽÍDEK (203 Czech Republic, belonging to the institution), Jan HEJÁTKO (203 Czech Republic, belonging to the institution) and Lubomír JANDA (203 Czech Republic, guarantor, belonging to the institution).
Edition The Plant Journal, England, BLACKWELL PUBLISHING, 2011, 0960-7412.
Other information
Original language English
Type of outcome Article in a journal
Field of Study Genetics and molecular biology
Country of publisher United Kingdom of Great Britain and Northern Ireland
Confidentiality degree is not subject to a state or trade secret
WWW URL
Impact factor Impact factor: 6.160
RIV identification code RIV/00216224:14740/11:00050106
Organization unit Central European Institute of Technology
Doi http://dx.doi.org/10.1111/j.1365-313X.2011.04637.x
UT WoS 000294827700008
Keywords in English multistep phosphorelay; receiver domain; CKI1; crystal structure; NMR spectroscopy; Arabidopsis thaliana
Tags ok, rivok
Tags International impact, Reviewed
Changed by Changed by: doc. RNDr. Jaromír Marek, Ph.D., učo 1989. Changed: 1/5/2012 01:49.
Abstract
Multistep phosphorelay (MSP) signaling mediates responses to a variety of important stimuli in plants. In Arabidopsis MSP, the signal is transferred from sensor histidine kinase (HK) via histidine phosphotransfer proteins (AHP1–AHP5) to nuclear response regulators. In contrast to ancestral two-component signaling in bacteria, protein interactions in plant MSP are supposed to be rather nonspecific. Here, we show that the C-terminal receiver domain of HK CKI1 (CKI1RD) is responsible for the recognition of CKI1 downstream signaling partners, and specifically interacts with AHP2, AHP3 and AHP5 with different affinities. We studied the effects of Mg2+, the co-factor necessary for signal transduction via MSP, and phosphorylation-mimicking BeF3 ) on CKI1RD in solution, and determined the crystal structure of free CKI1RD and CKI1RD in a complex with Mg2+. We found that the structure of CKI1RD shares similarities with the only known structure of plant HK, ETR1RD, with the main differences being in loop L3.
Links
ED1.1.00/02.0068, research and development projectName: CEITEC - central european institute of technology
GAP305/11/0756, research and development projectName: Strukturní princip specificity přenosu signálu v rostlinách: síť interakcí příjímačové domény histidinkinas u Arabidospis (Acronym: HKRD-AHP)
Investor: Czech Science Foundation
GA521/09/1699, research and development projectName: Imunomodulace jako nástroj funkční proteomiky při studiu cytokininové signální dráhy u Arabidopsis thaliana
Investor: Czech Science Foundation, Immunomodulation as a functional proteomics tool for cytokinin signaling study in Arabidopsis thaliana
GD204/08/H054, research and development projectName: Molekulární mechanismy proliferace a diferenciace buněk
Investor: Czech Science Foundation, Molecular mechanisms of the cell proliferation and differentiation
LC06034, research and development projectName: Regulace morfogeneze rostlinných buněk a orgánů
Investor: Ministry of Education, Youth and Sports of the CR, Regulation of morphogenesis of plant cells and organs
MSM0021622413, plan (intention)Name: Proteiny v metabolismu a při interakci organismů s prostředím
Investor: Ministry of Education, Youth and Sports of the CR, Proteins in metabolism and interaction of organisms with the environment
MSM0021622415, plan (intention)Name: Molekulární podstata buněčných a tkáňových regulací
Investor: Ministry of Education, Youth and Sports of the CR, Molecular basis of cell and tissue regulations
MUNI/A/0928/2009, interní kód MUName: Struktura a dynamika biopolymerů (Acronym: SDB)
Investor: Masaryk University, Category A
205872, interní kód MUName: Program developing interdisciplinary research POtential for the STudies of BIOMolecular INteractions (Acronym: POSTBIOMIN)
Investor: European Union, Program developing interdisciplinary research POtential for the STudies of BIOMolecular INteractions, Capacities
228461, interní kód MUName: Enhancing Access and Services to East European users Towards an efficient (Acronym: EAST-NMR)
Investor: European Union, Enhancing Access and Services to East European users Towards an efficient, Capacities
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