Structure and binding specificity of the receiver domain of sensor histidine kinase CKI1 from Arabidopsis thaliana.

PEKÁROVÁ, Blanka, Tomáš KLUMPLER, Olga TŘÍSKOVÁ, Jakub HORÁK, Séverine JANSEN, Radka DOPITOVÁ, Petra BORKOVCOVÁ, Veronika PAPOUŠKOVÁ, Eliška NEJEDLÁ, Vladimír SKLENÁŘ, Jaromír MAREK, Lukáš ŽÍDEK, Jan HEJÁTKO and Lubomír JANDA. Structure and binding specificity of the receiver domain of sensor histidine kinase CKI1 from Arabidopsis thaliana. The Plant Journal. England: BLACKWELL PUBLISHING, vol. 67, No 5, p. 827-839. ISSN 0960-7412. doi:10.1111/j.1365-313X.2011.04637.x. 2011.

Basic information

• Original name: Structure and binding specificity of the receiver domain of sensor histidine kinase CKI1 from Arabidopsis thaliana.
• Authors: PEKÁROVÁ, Blanka (203 Czech Republic, belonging to the institution), Tomáš KLUMPLER (203 Czech Republic, belonging to the institution), Olga TŘÍSKOVÁ (203 Czech Republic, belonging to the institution), Jakub HORÁK (203 Czech Republic, belonging to the institution), Séverine JANSEN (250 France, belonging to the institution), Radka DOPITOVÁ (203 Czech Republic, belonging to the institution), Petra BORKOVCOVÁ (203 Czech Republic, belonging to the institution), Veronika PAPOUŠKOVÁ (203 Czech Republic, belonging to the institution), Eliška NEJEDLÁ (203 Czech Republic, belonging to the institution), Vladimír SKLENÁŘ (203 Czech Republic, belonging to the institution), Jaromír MAREK (203 Czech Republic, belonging to the institution), Lukáš ŽÍDEK (203 Czech Republic, belonging to the institution), Jan HEJÁTKO (203 Czech Republic, belonging to the institution) and Lubomír JANDA (203 Czech Republic, guarantor, belonging to the institution).
• Edition: The Plant Journal, England, BLACKWELL PUBLISHING, 2011, 0960-7412.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: Genetics and molecular biology
• Country of publisher: United Kingdom of Great Britain and Northern Ireland
• Confidentiality degree: is not subject to a state or trade secret
• WWW: URL
• Impact factor: Impact factor: 6.160
• RIV identification code: RIV/00216224:14740/11:00050106
• Organization unit: Central European Institute of Technology
• Doi: http://dx.doi.org/10.1111/j.1365-313X.2011.04637.x
• UT WoS: 000294827700008
• Keywords in English: multistep phosphorelay; receiver domain; CKI1; crystal structure; NMR spectroscopy; Arabidopsis thaliana
• Tags: ok, rivok
• Tags: International impact, Reviewed

Abstract

Multistep phosphorelay (MSP) signaling mediates responses to a variety of important stimuli in plants. In Arabidopsis MSP, the signal is transferred from sensor histidine kinase (HK) via histidine phosphotransfer proteins (AHP1–AHP5) to nuclear response regulators. In contrast to ancestral two-component signaling in bacteria, protein interactions in plant MSP are supposed to be rather nonspecific. Here, we show that the C-terminal receiver domain of HK CKI1 (CKI1RD) is responsible for the recognition of CKI1 downstream signaling partners, and specifically interacts with AHP2, AHP3 and AHP5 with different affinities. We studied the effects of Mg2+, the co-factor necessary for signal transduction via MSP, and phosphorylation-mimicking BeF3 ) on CKI1RD in solution, and determined the crystal structure of free CKI1RD and CKI1RD in a complex with Mg2+. We found that the structure of CKI1RD shares similarities with the only known structure of plant HK, ETR1RD, with the main differences being in loop L3.

Links

• ED1.1.00/02.0068, research and development project: Name: CEITEC - central european institute of technology
• GAP305/11/0756, research and development project: Name: Strukturní princip specificity přenosu signálu v rostlinách: síť interakcí příjímačové domény histidinkinas u Arabidospis (Acronym: HKRD-AHP)

Investor: Czech Science Foundation

• GA521/09/1699, research and development project: Name: Imunomodulace jako nástroj funkční proteomiky při studiu cytokininové signální dráhy u Arabidopsis thaliana

Investor: Czech Science Foundation, Immunomodulation as a functional proteomics tool for cytokinin signaling study in Arabidopsis thaliana

• GD204/08/H054, research and development project: Name: Molekulární mechanismy proliferace a diferenciace buněk

Investor: Czech Science Foundation, Molecular mechanisms of the cell proliferation and differentiation

• LC06034, research and development project: Name: Regulace morfogeneze rostlinných buněk a orgánů

Investor: Ministry of Education, Youth and Sports of the CR, Regulation of morphogenesis of plant cells and organs

• MSM0021622413, plan (intention): Name: Proteiny v metabolismu a při interakci organismů s prostředím

Investor: Ministry of Education, Youth and Sports of the CR, Proteins in metabolism and interaction of organisms with the environment

• MSM0021622415, plan (intention): Name: Molekulární podstata buněčných a tkáňových regulací

Investor: Ministry of Education, Youth and Sports of the CR, Molecular basis of cell and tissue regulations

• MUNI/A/0928/2009, interní kód MU: Name: Struktura a dynamika biopolymerů (Acronym: SDB)

Investor: Masaryk University, Category A

• 205872, interní kód MU: Name: Program developing interdisciplinary research POtential for the STudies of BIOMolecular INteractions (Acronym: POSTBIOMIN)

Investor: European Union, Program developing interdisciplinary research POtential for the STudies of BIOMolecular INteractions, Capacities

• 228461, interní kód MU: Name: Enhancing Access and Services to East European users Towards an efficient (Acronym: EAST-NMR)

Investor: European Union, Enhancing Access and Services to East European users Towards an efficient, Capacities