The X-ray structure versus NMR structure of delta-subunit of RNA-polymerase

DEMO, Gabriel, Veronika PAPOUŠKOVÁ, Hana SANDEROVA, Lukáš ŽÍDEK and Michaela WIMMEROVÁ. The X-ray structure versus NMR structure of delta-subunit of RNA-polymerase. In XV. Setkani biochemiku a molekularnich biologu. 2011. ISBN 978-80-210-5594-0.

Basic information

Original name

The X-ray structure versus NMR structure of delta-subunit of RNA-polymerase

Name in Czech

Roetgenova struktura verzus NMR struktura delta-podjednotky RNA-polymerazy

Authors

DEMO, Gabriel (703 Slovakia, belonging to the institution), Veronika PAPOUŠKOVÁ (203 Czech Republic, belonging to the institution), Hana SANDEROVA (203 Czech Republic), Lukáš ŽÍDEK (203 Czech Republic, belonging to the institution) and Michaela WIMMEROVÁ (203 Czech Republic, guarantor, belonging to the institution)

Edition

XV. Setkani biochemiku a molekularnich biologu, 2011

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Other information

Language

English

Type of outcome

Konferenční abstrakt

Field of Study

10600 1.6 Biological sciences

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14740/11:00050113

Organization unit

Central European Institute of Technology

ISBN

978-80-210-5594-0

Keywords (in Czech)

X-ray NMR struktura delta-podjednotka porovnani

Keywords in English

X-ray NMR structure delta-subunit comparison

Tags

rivok

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Abstract

V originále

The N-terminal domain of delta subunit was conquested to high-throughput screening. Iodine compound was used as a co-crystalline substance to have a possibility to provide a Single Anomalous Dispersion (SAD) experiments. Diffraction data were collected at BESSY II Berlin, beamline MX-14.2. The alpha-helical part of the protein is in a good agreement with the NMR structure. In the region of beta-sheets for the NMR structure the X-ray structure showed completely different behaviour (folding).

In Czech

N-terminalni domena delta podjednotky byla podrobena siroke krystalizaci na urcei vhodnych krystalizacnich podminek. Jodova sloucenina byla pouzita jako ko-krystalizacni latka kvuli uskutocneni SAD experimentu (anomalni rozptyl). Difrakcni data byli zozbirany na BESSY II v Berlinu na stanovisti MX-14.2. Alfa-helikalni cast proteinu je v souladu s NMR strukturou tohto proteinu. Avsak v oblasti beta-listu ktere byli urceny NMR ukazala roetgenova struktura uplne jine chovani v usporadani.

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Links

GA204/09/0583, research and development project	Name: Delta podjednotka RNA polymerázy z Gram pozitivních bakterií (Acronym: DELTA) Investor: Czech Science Foundation
MSM0021622413, plan (intention)	Name: Proteiny v metabolismu a při interakci organismů s prostředím Investor: Ministry of Education, Youth and Sports of the CR, Proteins in metabolism and interaction of organisms with the environment