The X-ray structure versus NMR structure of delta-subunit of RNA-polymerase
DEMO, Gabriel, Veronika PAPOUŠKOVÁ, Hana SANDEROVA, Lukáš ŽÍDEK and Michaela WIMMEROVÁ. The X-ray structure versus NMR structure of delta-subunit of RNA-polymerase. In XV. Setkani biochemiku a molekularnich biologu. 2011. ISBN 978-80-210-5594-0. |
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Original name | The X-ray structure versus NMR structure of delta-subunit of RNA-polymerase |
Name in Czech | Roetgenova struktura verzus NMR struktura delta-podjednotky RNA-polymerazy |
Authors | DEMO, Gabriel (703 Slovakia, belonging to the institution), Veronika PAPOUŠKOVÁ (203 Czech Republic, belonging to the institution), Hana SANDEROVA (203 Czech Republic), Lukáš ŽÍDEK (203 Czech Republic, belonging to the institution) and Michaela WIMMEROVÁ (203 Czech Republic, guarantor, belonging to the institution). |
Edition | XV. Setkani biochemiku a molekularnich biologu, 2011. |
Other information | |
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Original language | English |
Type of outcome | Conference abstract |
Field of Study | 10600 1.6 Biological sciences |
Country of publisher | Czech Republic |
Confidentiality degree | is not subject to a state or trade secret |
RIV identification code | RIV/00216224:14740/11:00050113 |
Organization unit | Central European Institute of Technology |
ISBN | 978-80-210-5594-0 |
Keywords (in Czech) | X-ray NMR struktura delta-podjednotka porovnani |
Keywords in English | X-ray NMR structure delta-subunit comparison |
Tags | rivok |
Changed by | Changed by: Mgr. Nikola Kostlánová, Ph.D., učo 12689. Changed: 4/4/2012 12:09. |
Abstract |
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The N-terminal domain of delta subunit was conquested to high-throughput screening. Iodine compound was used as a co-crystalline substance to have a possibility to provide a Single Anomalous Dispersion (SAD) experiments. Diffraction data were collected at BESSY II Berlin, beamline MX-14.2. The alpha-helical part of the protein is in a good agreement with the NMR structure. In the region of beta-sheets for the NMR structure the X-ray structure showed completely different behaviour (folding). |
Abstract (in Czech) |
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N-terminalni domena delta podjednotky byla podrobena siroke krystalizaci na urcei vhodnych krystalizacnich podminek. Jodova sloucenina byla pouzita jako ko-krystalizacni latka kvuli uskutocneni SAD experimentu (anomalni rozptyl). Difrakcni data byli zozbirany na BESSY II v Berlinu na stanovisti MX-14.2. Alfa-helikalni cast proteinu je v souladu s NMR strukturou tohto proteinu. Avsak v oblasti beta-listu ktere byli urceny NMR ukazala roetgenova struktura uplne jine chovani v usporadani. |
Links | |
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GA204/09/0583, research and development project | Name: Delta podjednotka RNA polymerázy z Gram pozitivních bakterií (Acronym: DELTA) |
Investor: Czech Science Foundation | |
MSM0021622413, plan (intention) | Name: Proteiny v metabolismu a při interakci organismů s prostředím |
Investor: Ministry of Education, Youth and Sports of the CR, Proteins in metabolism and interaction of organisms with the environment |
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