a 2011

Polyethyleneimine coated capillary in nucleotide and short oligonucleotide analysis

BITTOVÁ, Miroslava, Vendula ROBLOVÁ, Eva MAJEROVÁ and Libuše TRNKOVÁ

Basic information

Original name

Polyethyleneimine coated capillary in nucleotide and short oligonucleotide analysis

Authors

BITTOVÁ, Miroslava (203 Czech Republic, guarantor, belonging to the institution), Vendula ROBLOVÁ (203 Czech Republic, belonging to the institution), Eva MAJEROVÁ (203 Czech Republic, belonging to the institution) and Libuše TRNKOVÁ (203 Czech Republic, belonging to the institution)

Edition

8th International Interdisciplinary Meeting on Bioanalysis - CECE 2011, 2011

Other information

Language

English

Type of outcome

Konferenční abstrakt

Field of Study

10406 Analytical chemistry

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14310/11:00053816

Organization unit

Faculty of Science

ISBN

978-80-904959-0-6

Keywords in English

capillary electrophoresis polyethyleneimine coating nucleotides oligonucleotides

Tags

Změněno: 13/4/2012 12:40, prof. RNDr. Libuše Trnková, CSc.

Abstract

V originále

Inner surface modification of fused-silica capillaries in capillary zone electrophoresis with an appropriate chemical reagents allow targeted selection of separation system for analytes of interest. Polyethyleneimine (PEI) is polycationic highly branched polymer with positively charged primary, secondary and tertiary amino groups in the structure that can be used as fused-silica capillary modifier. Its irreversible adsorption on the silanol groups of the fused-silica capillary surface eliminates negative charge and excess of charged amino groups cause reversion of electroosmotic flow. In our work, we successfully applied polyethyleneimine coated capillaries in analysis of nucleotides and synthetic pentamers. We studied the influence of particular component of Tris/boric acid/EDTA buffer (TBE) on the PEI coated capillary surface and the effect of hydroxyethylcellulose (HEC) and/or B-cyclodextrin on the nucleotide separation. Finally, we analyzed DNA restricted fragments from Nicotiana tabacum.

Links

LC06035, research and development project
Name: Centrum biofyzikální chemie, bioelektrochemie a bioanalýzy. Nové nástroje pro genomiku, proteomiku a biomedicínu.
Investor: Ministry of Education, Youth and Sports of the CR, Centre of Biophysical Chemistry, Bioelectrochemistry and Bioanalysis. New Tools for Genomics, Proteomics and Biomedicine