Detailed Information on Publication Record
2011
Polyethyleneimine coated capillary in nucleotide and short oligonucleotide analysis
BITTOVÁ, Miroslava, Vendula ROBLOVÁ, Eva MAJEROVÁ and Libuše TRNKOVÁBasic information
Original name
Polyethyleneimine coated capillary in nucleotide and short oligonucleotide analysis
Authors
BITTOVÁ, Miroslava (203 Czech Republic, guarantor, belonging to the institution), Vendula ROBLOVÁ (203 Czech Republic, belonging to the institution), Eva MAJEROVÁ (203 Czech Republic, belonging to the institution) and Libuše TRNKOVÁ (203 Czech Republic, belonging to the institution)
Edition
8th International Interdisciplinary Meeting on Bioanalysis - CECE 2011, 2011
Other information
Language
English
Type of outcome
Konferenční abstrakt
Field of Study
10406 Analytical chemistry
Country of publisher
Czech Republic
Confidentiality degree
není předmětem státního či obchodního tajemství
RIV identification code
RIV/00216224:14310/11:00053816
Organization unit
Faculty of Science
ISBN
978-80-904959-0-6
Keywords in English
capillary electrophoresis polyethyleneimine coating nucleotides oligonucleotides
Tags
Změněno: 13/4/2012 12:40, prof. RNDr. Libuše Trnková, CSc.
Abstract
V originále
Inner surface modification of fused-silica capillaries in capillary zone electrophoresis with an appropriate chemical reagents allow targeted selection of separation system for analytes of interest. Polyethyleneimine (PEI) is polycationic highly branched polymer with positively charged primary, secondary and tertiary amino groups in the structure that can be used as fused-silica capillary modifier. Its irreversible adsorption on the silanol groups of the fused-silica capillary surface eliminates negative charge and excess of charged amino groups cause reversion of electroosmotic flow. In our work, we successfully applied polyethyleneimine coated capillaries in analysis of nucleotides and synthetic pentamers. We studied the influence of particular component of Tris/boric acid/EDTA buffer (TBE) on the PEI coated capillary surface and the effect of hydroxyethylcellulose (HEC) and/or B-cyclodextrin on the nucleotide separation. Finally, we analyzed DNA restricted fragments from Nicotiana tabacum.
Links
LC06035, research and development project |
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