Analysis of Biomolecules through Surface-Assisted Laser Desorption/Ionization Mass Spectrometry Employing Nanomaterials

CHEN, Wen-Tsen, Iva TOMALOVÁ, Jan PREISLER and Huan-Tsung CHANG. Analysis of Biomolecules through Surface-Assisted Laser Desorption/Ionization Mass Spectrometry Employing Nanomaterials. Journal of the Chinese Chemical Society. Taipei, 2011, vol. 58, No 6, p. 769-778. ISSN 0009-4536. Available from: https://dx.doi.org/10.1002/jccs.201190120.

Basic information

• Original name: Analysis of Biomolecules through Surface-Assisted Laser Desorption/Ionization Mass Spectrometry Employing Nanomaterials
• Name in Czech: Analýza Biomolekul Hmotnostní spektrometrií s laserovou desorpcí za účasti nanomateriálů
• Authors: CHEN, Wen-Tsen (158 Taiwan), Iva TOMALOVÁ (203 Czech Republic, belonging to the institution), Jan PREISLER (203 Czech Republic, guarantor, belonging to the institution) and Huan-Tsung CHANG (158 Taiwan).
• Edition: Journal of the Chinese Chemical Society, Taipei, 2011, 0009-4536.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: 10406 Analytical chemistry
• Country of publisher: Taiwan
• Confidentiality degree: is not subject to a state or trade secret
• Impact factor: Impact factor: 0.678
• RIV identification code: RIV/00216224:14740/11:00050122
• Organization unit: Central European Institute of Technology
• Doi: http://dx.doi.org/10.1002/jccs.201190120
• UT WoS: 000296267600009
• Keywords (in Czech): Nanomateriály, Biomolekuly, Hmotnostní spektrometrie s laserovou desorpcí za účasti povrchu, Zobrazovací hmotnostní spektrometrie
• Keywords in English: Nanomaterials; Biomolecules; Surface-assisted laser desorption/ionization mass spectrometry; Mass spectrometry imaging
• Tags: ok, rivok
• Tags: Reviewed

Abstract

Nanomaterials, primarily nanoparticles (NPs), can serve as an alternative matrix for the analysis of various biomolecules through SALDI MS. SALDI MS has been developed to overcome poor reproducibility and high background in the low mass region commonly occurring in MALDI MS. Various nanomaterials, unmodified or functionalized with recognition ligands, can have a strong affinity toward certain analytes and thus are applicable for their concentration and enrichment from complex biological matrices. In mass spectrometry imaging (MSI), the use of NPs instead of the conventional matrices can improve the spatial resolution up to the cellular level. In this review, the nature of NPs, the methods of sample preparation and approaches for quantitation of biomolecules through SALDI MS are discussed. Practical applications and limitations of SALDI MS employing NPs for separate samples and MSI are mentioned. With regard to the nature of MSI analysis, the use of nanostructured surfaces for MSI is also reflected in this review.

Links

• ED1.1.00/02.0068, research and development project: Name: CEITEC - central european institute of technology
• GCP206/10/J012, research and development project: Name: Analýza biomolekul hmotnostní spektrometrií s laserovou desorpcí/ionizací za účasti nanomateriálů

Investor: Czech Science Foundation

• MSM0021622415, plan (intention): Name: Molekulární podstata buněčných a tkáňových regulací

Investor: Ministry of Education, Youth and Sports of the CR, Molecular basis of cell and tissue regulations

• MUNI/A/0992/2009, interní kód MU: Name: Vývoj metodologie a instrumentace analytické chemie pro studium složení a vlastností přírodních a syntetických materiálů (Acronym: MIA)

Investor: Masaryk University, Category A