| MICHALCOVÁ, Lenka, Radka DOPITOVÁ, Jan HEJÁTKO, Markéta RYVOLOVÁ, Martina VÁLKOVÁ a Jan PREISLER. CE-LIF of Green Fluorescent Fusion Proteins for Investigation of Phosphorylation. In 8 th International Interdisciplinary Meeting on Bioanalysis, CECE 2011. ISBN 978-80-904959-0-6. 2011. |
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@proceedings{956990,
author = {Michalcová, Lenka and Dopitová, Radka and Hejátko, Jan and Ryvolová, Markéta and Válková, Martina and Preisler, Jan},
booktitle = {8 th International Interdisciplinary Meeting on Bioanalysis, CECE 2011},
keywords = {CE-LIF; phosphorylation; AHP proteins},
language = {eng},
isbn = {978-80-904959-0-6},
title = {CE-LIF of Green Fluorescent Fusion Proteins for Investigation of Phosphorylation},
year = {2011}
}
TY - CONF
ID - 956990
AU - Michalcová, Lenka - Dopitová, Radka - Hejátko, Jan - Ryvolová, Markéta - Válková, Martina - Preisler, Jan
PY - 2011
TI - CE-LIF of Green Fluorescent Fusion Proteins for Investigation of Phosphorylation
SN - 9788090495906
KW - CE-LIF
KW - phosphorylation
KW - AHP proteins
N2 - In this study, CE-LIF with 405 nm laser diode was used to study AHP5 fused with GFP. AHP5-GFP fusion proteins overexpressed in E. coli were used for initial optimization of CE-LIF technique because of lower concentrations of the fusion protein produced by plant. The stability of the AHP5-GFP sample was examined; the complex was found to be unstable with GFP being released. The GFP release was not affected by either a long-term storage at -80 and -20 C or the addition of one of the inhibitors (EDTA, PMSF). These results were confirmed by the western blotting. The phosphorylation of the sample AHP5-GFP in vitro is under investigation. Gel filtration was used to obtain mass fractions for better understanding electrophoregrams. The mass fractions were investigated by CE-LIF and Western blotting-LIF and Western blotting.
ER -
MICHALCOVÁ, Lenka, Radka DOPITOVÁ, Jan HEJÁTKO, Markéta RYVOLOVÁ, Martina VÁLKOVÁ a Jan PREISLER. CE-LIF of Green Fluorescent Fusion Proteins for Investigation of Phosphorylation. In \textit{8 th International Interdisciplinary Meeting on Bioanalysis, CECE 2011}. ISBN~978-80-904959-0-6. 2011.
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