Enhancement of AhR-mediated activity of selected pollutants and their mixtures after interaction with dissolved organic matter

BITTNER, Michal, Petra MACÍKOVÁ, John P. GIESY and Klára HILSCHEROVÁ. Enhancement of AhR-mediated activity of selected pollutants and their mixtures after interaction with dissolved organic matter. Environment International. OXFORD, ENGLAND: PERGAMON-ELSEVIER, 2011, vol. 37, No 5, p. 960-964. ISSN 0160-4120. Available from: https://dx.doi.org/10.1016/j.envint.2011.03.016.

Basic information

• Original name: Enhancement of AhR-mediated activity of selected pollutants and their mixtures after interaction with dissolved organic matter
• Authors: BITTNER, Michal (203 Czech Republic, belonging to the institution), Petra MACÍKOVÁ (203 Czech Republic, belonging to the institution), John P. GIESY (124 Canada) and Klára HILSCHEROVÁ (203 Czech Republic, guarantor, belonging to the institution).
• Edition: Environment International, OXFORD, ENGLAND, PERGAMON-ELSEVIER, 2011, 0160-4120.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: 30304 Public and environmental health
• Country of publisher: United Kingdom of Great Britain and Northern Ireland
• Confidentiality degree: is not subject to a state or trade secret
• Impact factor: Impact factor: 5.297
• RIV identification code: RIV/00216224:14310/11:00050132
• Organization unit: Faculty of Science
• Doi: http://dx.doi.org/10.1016/j.envint.2011.03.016
• UT WoS: 000292012100017
• Keywords in English: Humic substances; AhR; Mixture; Interaction; H4IIE-luc; Organic pollutants
• Tags: AKR, rivok

Abstract

In vitro effects of binary mixtures of various DOM (mainly HS) with various HOCs on AhR-mediated responses were studied by use of H4IIE-luc cells. 6 out of 12 DOM activated the AhR even at environmentally relevant concentrations (17 mg L(-1)). In simultaneous exposures of H4IIE-luc cells to DOM and each of the model compounds, 2,3,7,8-TCDD, PCB126, PCB169, benzo[a]pyrene, benzo[a]anthracene, dibenz[a,h]anthracene, fluoranthene, a mixture of POPs, a mixture of PAHs, and a mixture of all HOCs, either significant additive or facilitative effects were observed when compared to activities of single HOCs. No significant decrease of effects due to sorption of HOCs to DOM was observed, even in subsequent experiments when HOCs + DOM mixtures were preincubated for six days before exposure to H4IIE-luc. Thus, DOM does not seem to protect organisms against AhR-mediated toxic effects of HOCs (as predicted due to sorption of HOCs on DOM), but it can enhance their potency for AhR-mediated effects in some situations.

Links

• ED0001/01/01, research and development project: Name: CETOCOEN
• GP525/08/P464, research and development project: Name: Interakce huminových látek a UV-VIS záření s dioxinově aktivními environmentálními polutanty

Investor: Czech Science Foundation, Interaction of humic substances and UV-VIS irradiation with dioxin-like environmental pollutants

• MSM0021622412, plan (intention): Name: Interakce mezi chemickými látkami, prostředím a biologickými systémy a jejich důsledky na globální, regionální a lokální úrovni (INCHEMBIOL) (Acronym: INCHEMBIOL)

Investor: Ministry of Education, Youth and Sports of the CR, Interactions among the chemicals, environment and biological systems and their consequences on the global, regional and local scales (INCHEMBIOL)