c-Myb regulates MMP1, MMP9 and cathepsin D: Implications for the matrix-dependent breast cancer cell invasion and metastasis

KNOPFOVÁ, Lucia, Petr BENEŠ, Lucie PEKARČÍKOVÁ, Markéta HERMANOVÁ, Michal MASAŘÍK, Karel SOUČEK and Jan ŠMARDA. c-Myb regulates MMP1, MMP9 and cathepsin D: Implications for the matrix-dependent breast cancer cell invasion and metastasis. In Zing Cancer Conference. 2011.

Basic information

• Original name: c-Myb regulates MMP1, MMP9 and cathepsin D: Implications for the matrix-dependent breast cancer cell invasion and metastasis
• Authors: KNOPFOVÁ, Lucia (203 Czech Republic, belonging to the institution), Petr BENEŠ (203 Czech Republic, belonging to the institution), Lucie PEKARČÍKOVÁ (203 Czech Republic, belonging to the institution), Markéta HERMANOVÁ (203 Czech Republic, belonging to the institution), Michal MASAŘÍK (203 Czech Republic, belonging to the institution), Karel SOUČEK (203 Czech Republic, belonging to the institution) and Jan ŠMARDA (203 Czech Republic, guarantor, belonging to the institution).
• Edition: Zing Cancer Conference, 2011.

Other information

• Original language: English
• Type of outcome: Conference abstract
• Field of Study: Genetics and molecular biology
• Country of publisher: Mexico
• Confidentiality degree: is not subject to a state or trade secret
• RIV identification code: RIV/00216224:14310/11:00049492
• Organization unit: Faculty of Science
• Keywords in English: c-Myb; breast cancer; cell invasion; metastasis

Abstract

Ectopically expressed c-Myb enhanced motility and ability of human MDA-MB-231 and mouse 4T1 mammary cancer cells to invade Matrigel but not the collagen I matrix in vitro. c-Myb strongly increased expression/activity of cathepsin D and matrix metalloproteinase (MMP) 9 and significantly down-regulated MMP1. The gene coding for cathepsin D was identified as direct c-Myb-target gene and downstream effector of the c-Myb migration-promoting function. Finally, we demonstrated that c-Myb delayed growth of mammary tumors in BALB/c mice and affected metastatic potential of breast cancer cells in an organ-specific manner. This study demonstrates potential role of a stromal context for the c-Myb function in control of breast cancer invasion and metastasis.

Links

• GA301/09/1115, research and development project: Name: Úloha proteinů c-Myb a Cox-2 při tvorbě střevních nádorů

Investor: Czech Science Foundation, The role of c-Myb and Cox-2 proteins in colon carcinogenesis

• GD204/08/H054, research and development project: Name: Molekulární mechanismy proliferace a diferenciace buněk

Investor: Czech Science Foundation, Molecular mechanisms of the cell proliferation and differentiation

• IAA501630801, research and development project: Name: Mechanismus účinku prokatepsinu D na buňky rakoviny prsu

Investor: Academy of Sciences of the Czech Republic, Mechanism of procathepsin D effect on breast cancer cells

• MSM0021622415, plan (intention): Name: Molekulární podstata buněčných a tkáňových regulací

Investor: Ministry of Education, Youth and Sports of the CR, Molecular basis of cell and tissue regulations