Identification of Bifidobacterium spp. isolated from children intestinal mucous tissue samples by MALDI-TOF MS and automated ribotyping

ŠVEC, Pavel, Ondrej ŠEDO, Andrea TESHIM, Vladimír DRÁB, Zbyněk ZDRÁHAL and Ivo SEDLÁČEK. Identification of Bifidobacterium spp. isolated from children intestinal mucous tissue samples by MALDI-TOF MS and automated ribotyping. In 4th Congress of European Microbiologists FEMS 2011. 2011.

Basic information

• Original name: Identification of Bifidobacterium spp. isolated from children intestinal mucous tissue samples by MALDI-TOF MS and automated ribotyping
• Authors: ŠVEC, Pavel (203 Czech Republic, guarantor, belonging to the institution), Ondrej ŠEDO (203 Czech Republic, belonging to the institution), Andrea TESHIM (203 Czech Republic, belonging to the institution), Vladimír DRÁB (203 Czech Republic), Zbyněk ZDRÁHAL (203 Czech Republic, belonging to the institution) and Ivo SEDLÁČEK (203 Czech Republic, belonging to the institution).
• Edition: 4th Congress of European Microbiologists FEMS 2011, 2011.

Other information

• Original language: English
• Type of outcome: Conference abstract
• Field of Study: 10600 1.6 Biological sciences
• Country of publisher: Czech Republic
• Confidentiality degree: is not subject to a state or trade secret
• RIV identification code: RIV/00216224:14310/11:00054000
• Organization unit: Faculty of Science
• Keywords (in Czech): Bifodobacterium; taxonomie; identifikace
• Keywords in English: Bifodobacterium; taxonomy; identification

Abstract

Background: Bifidobacteria represent an important part of human gastrointestinal tract maintaining and balancing proper intestinal microflora. Their correct identification is crucial for taxonomical, epidemiological and clinical purposes. Objectives: The aim of this work was to evaluate capabilities of automated ribotyping for identification of bifidobacterial isolates from human intestine. Methods: A set of 70 Bifidobacterium strains originating from intestinal mucous tissue samples from children patients was identified by MALDI-TOF MS performed on an Ultraflex III instrument (Bruker Daltonik) and further characterized using automated ribotyping performed by the RiboPrinter system (DuPont Qualicon). Results: MALDI-TOF MS analysis enabled reliable identification (BioTyper log(score) > 2.3) of Bifidobacterium longum (36 strains), Bifidobacterium adolescentis (23), Bifidobacterium catenulatum (7), Bifidobacterium breve (3) and Bifidobacterium animalis (1). The RiboPrinter system assigned only 33 strains to the species level and identified B. longum (18 strains), B. adolescentis (10), B. breve (3) and single representatives of B. animalis and Bifidobacterium boum. These outcomes were congruent with MALDI-TOF MS results except for two B. adolescentis strains which were identified by ribotyping as B. longum and B. boum, respectively, and six B. catenulatum strains assigned as B. adolescentis. The remaining isolates were not identified as they did not exceed the threshold of 85 % similarity with the RiboPrinter reference database entries. Conclusions: The RiboPrinter system, which identified correctly only 35 % of analysed strains, has been shown as an unsuitable tool for identification of Bifidobacterium spp. occurring in human intestines. This work was supported by projects 2B08068, MSM0021622415, MSM0021622416, and LC06034.

Links

• LC06034, research and development project: Name: Regulace morfogeneze rostlinných buněk a orgánů

Investor: Ministry of Education, Youth and Sports of the CR, Regulation of morphogenesis of plant cells and organs

• MSM0021622415, plan (intention): Name: Molekulární podstata buněčných a tkáňových regulací

Investor: Ministry of Education, Youth and Sports of the CR, Molecular basis of cell and tissue regulations

• MSM0021622416, plan (intention): Name: Diverzita biotických společenstev a populací: kauzální analýza variability v prostoru a čase

Investor: Ministry of Education, Youth and Sports of the CR, Diversity of Biotic Communities and Populations: Causal Analysis of variation in space and time

• 2B08068, research and development project: Name: Výzkum možností aplikace automatizovaných a poloautomatizovaných metod pro identifikaci a charakterizaci nových kmenů baktérií s probiotickými vlastnostmi s cílem rozšíření specifických genetických zdrojů pro prevenci civilizačních onemocnění. (Acronym: GASTROPROBIO)

Investor: Ministry of Education, Youth and Sports of the CR, Research into possibilities of application both automated and semi-automated methods for identification and describing new microorganisms with probiotic properties with aim to extend specific genetic sources for civilizing diseases prevention