Identification of Lactobacillus spp. isolates from children's intestinal tissues by MALDI-TOF MS profiling and automated ribotyping

TESHIM, Andrea, Ondrej ŠEDO, Pavel ŠVEC, Vladimír DRÁB, Ivo SEDLÁČEK and Zbyněk ZDRÁHAL. Identification of Lactobacillus spp. isolates from children's intestinal tissues by MALDI-TOF MS profiling and automated ribotyping. In 4th Congress of European Microbiologists FEMS 2011. 2011.

Basic information

• Original name: Identification of Lactobacillus spp. isolates from children's intestinal tissues by MALDI-TOF MS profiling and automated ribotyping
• Authors: TESHIM, Andrea (203 Czech Republic, guarantor, belonging to the institution), Ondrej ŠEDO (203 Czech Republic, belonging to the institution), Pavel ŠVEC (203 Czech Republic, belonging to the institution), Vladimír DRÁB (203 Czech Republic), Ivo SEDLÁČEK (203 Czech Republic, belonging to the institution) and Zbyněk ZDRÁHAL (203 Czech Republic, belonging to the institution).
• Edition: 4th Congress of European Microbiologists FEMS 2011, 2011.

Other information

• Original language: English
• Type of outcome: Conference abstract
• Field of Study: 10600 1.6 Biological sciences
• Country of publisher: Czech Republic
• Confidentiality degree: is not subject to a state or trade secret
• RIV identification code: RIV/00216224:14310/11:00054001
• Organization unit: Faculty of Science
• Keywords in English: Lactobacillus; taxonomy; identification

Abstract

Background: In clinical microbiology as well as in dairy industry there is a great need for reliable identification and characterization of lactic acid bacteria. Matrix Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI TOF MS) represents a fast, procedurally simple and sensitive chemotaxonomical method with a high discriminatory power. The identification of bacteria is based on characteristic and reproducible peptide/protein fingerprints. Objectives: The aim of this work was to assess MALDI TOF MS profiling and automatic ribotyping as tools for reliable identification of Lactobacillus isolates from human intestinal tissues. Methods: MALDI-TOF mass spectra of 57 Lactobacillus strains were obtained with an Ultraflex III instrument (Bruker Daltonik). BioTyper software (Bruker Daltonik) involving a database of lactic acid bacteria MALDI-TOF MS profiles was used for data processing. Automated ribotyping was performed with a RiboPrinter identification system (DuPont Qualicon) including DuPont Qualicon database DUP 2008 provided by the manufacturer. Conclusions: Based on MALDI-TOF MS analysis, Lactobacillus salivarius (28 strains), Lactobacillus paracasei (11), Lactobacillus rhamnosus (7), Lactobacillus mucosae (5), Lactobacillus gasseri (2), Lactobacillus plantarum (2), Lactobacillus casei/paracasiei (1) and Lactobacillus oris (1) were reliably identified (BioTyper log(score) > 2.3). In accordance with MALDI-TOF MS, the RiboPrinter system identified Lactobacillus salivarius (19 strains), Lactobacillus paracasei (12) and Lactobacillus gasseri (1). One L. gasseri strain was assigned as Aerococcus viridans. The remaining strains were not identified using the ribotyping method. MALDI-TOF MS proved to be suitable tool for identification of Lactobacillus spp. inhabiting human intestinal tract. This work was supported by projects 2B08068, MSM0021622415, MSM0021622416 and LC06034.

Links

• LC06034, research and development project: Name: Regulace morfogeneze rostlinných buněk a orgánů

Investor: Ministry of Education, Youth and Sports of the CR, Regulation of morphogenesis of plant cells and organs

• MSM0021622415, plan (intention): Name: Molekulární podstata buněčných a tkáňových regulací

Investor: Ministry of Education, Youth and Sports of the CR, Molecular basis of cell and tissue regulations

• MSM0021622416, plan (intention): Name: Diverzita biotických společenstev a populací: kauzální analýza variability v prostoru a čase

Investor: Ministry of Education, Youth and Sports of the CR, Diversity of Biotic Communities and Populations: Causal Analysis of variation in space and time

• 2B08068, research and development project: Name: Výzkum možností aplikace automatizovaných a poloautomatizovaných metod pro identifikaci a charakterizaci nových kmenů baktérií s probiotickými vlastnostmi s cílem rozšíření specifických genetických zdrojů pro prevenci civilizačních onemocnění. (Acronym: GASTROPROBIO)

Investor: Ministry of Education, Youth and Sports of the CR, Research into possibilities of application both automated and semi-automated methods for identification and describing new microorganisms with probiotic properties with aim to extend specific genetic sources for civilizing diseases prevention