| ZÍTKA, Ondřej, Sona KŘÍŽKOVÁ, Ludmila KREJČOVÁ, David HYNEK, Jaromír GUMULEC, Michal MASAŘÍK, Jiří SOCHOR, Vojtěch ADAM, Jaromír HUBÁLEK, Libuše TRNKOVÁ and René KIZEK. Microfluidic tool based on the antibodymodified paramagnetic particles for detection of 8-hydroxy-20-deoxyguanosine in urine of prostate cancer patients. Electrophoresis. 2011, vol. 32, No 22, p. 3207–3220. ISSN 0173-0835. Available from: https://dx.doi.org/10.1002/elps.201100430. |
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@article{958943,
author = {Zítka, Ondřej and Křížková, Sona and Krejčová, Ludmila and Hynek, David and Gumulec, Jaromír and Masařík, Michal and Sochor, Jiří and Adam, Vojtěch and Hubálek, Jaromír and Trnková, Libuše and Kizek, René},
article_number = {22},
doi = {http://dx.doi.org/10.1002/elps.201100430},
keywords = {Electrochemistry; ELISA; Magnetic particles; Nanoseparation},
language = {eng},
issn = {0173-0835},
journal = {Electrophoresis},
title = {Microfluidic tool based on the antibodymodified paramagnetic particles for detection of 8-hydroxy-20-deoxyguanosine in urine of prostate cancer patients},
url = {http://onlinelibrary.wiley.com/doi/10.1002/elps.201100430/pdf},
volume = {32},
year = {2011}
}
TY - JOUR
ID - 958943
AU - Zítka, Ondřej - Křížková, Sona - Krejčová, Ludmila - Hynek, David - Gumulec, Jaromír - Masařík, Michal - Sochor, Jiří - Adam, Vojtěch - Hubálek, Jaromír - Trnková, Libuše - Kizek, René
PY - 2011
TI - Microfluidic tool based on the antibodymodified paramagnetic particles for detection of 8-hydroxy-20-deoxyguanosine in urine of prostate cancer patients
JF - Electrophoresis
VL - 32
IS - 22
SP - 3207–3220
EP - 3207–3220
SN - 01730835
KW - Electrochemistry
KW - ELISA
KW - Magnetic particles
KW - Nanoseparation
UR - http://onlinelibrary.wiley.com/doi/10.1002/elps.201100430/pdf
L2 - http://onlinelibrary.wiley.com/doi/10.1002/elps.201100430/pdf
N2 - Guanosine derivatives are important for diagnosis of oxidative DNA damage including 8-hydroxy-20-deoxyguanosine (8-OHdG) as one of the most abundant products of DNA oxidation. This compound is commonly determined in urine, which makes 8-OHdG a good non-invasive marker of oxidation stress. In this study, we optimized and tested the isolation of 8-OHdG from biological matrix by using paramagnetic particles with an antibody-modified surface. 8-OHdG was determined using 1-naphthol generated by alkaline phosphatase conjugated with the secondary antibody. 1-Naphthol was determined by stopped flow injection analysis (SFIA) with electrochemical detector using a glassy carbon working electrode and by stationary electrochemical detection using linear sweep voltammetry. A special modular electrochemical SFIA system which needs only 10 mL of sample including working buffer for one analysis was completely designed and successfully verified. The recoveries in different matrices and analyte concentration were estimated. Detection limit (3 S/N) was estimated as 5 pg/mL of 8-OHdG. This method promises to be very easily modified to microfluidic systems as ‘‘lab on valve’’. The optimized method had sufficient selectivity and thus could be used for determination of 8-OHDG in human urine and therefore for estimation of oxidative DNA damage as a result of oxidation stress in prostate cancer patients.
ER -
ZÍTKA, Ondřej, Sona KŘÍŽKOVÁ, Ludmila KREJČOVÁ, David HYNEK, Jaromír GUMULEC, Michal MASAŘÍK, Jiří SOCHOR, Vojtěch ADAM, Jaromír HUBÁLEK, Libuše TRNKOVÁ and René KIZEK. Microfluidic tool based on the antibodymodified paramagnetic particles for detection of 8-hydroxy-20-deoxyguanosine in urine of prostate cancer patients. \textit{Electrophoresis}. 2011, vol.~32, No~22, p.~3207–3220. ISSN~0173-0835. Available from: https://dx.doi.org/10.1002/elps.201100430.
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