MALIŠOVÁ, Barbora, Eva NĚMCOVÁ, Jiří POL, Z. ŠORM, Š. WURMOVÁ, K. NOVOTNÁ, M. VANĚRKOVÁ, Veronika HOLÁ, Filip RŮŽIČKA, Ladislav DUŠEK, Petr NĚMEC and Tomáš FREIBERGER. Value of PCR in surgically treated patients with staphylococcal infective endocarditis: a 4-year retrospective study. European Journal of Clinical Microbiology & Infectious Diseases. 2012, vol. 31, No 6, p. 1187-1194. ISSN 0934-9723. doi:10.1007/s10096-011-1427-3.
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Basic information
Original name Value of PCR in surgically treated patients with staphylococcal infective endocarditis: a 4-year retrospective study
Authors MALIŠOVÁ, Barbora (203 Czech Republic), Eva NĚMCOVÁ (203 Czech Republic), Jiří POL (203 Czech Republic), Z. ŠORM (203 Czech Republic), Š. WURMOVÁ (203 Czech Republic), K. NOVOTNÁ (203 Czech Republic), M. VANĚRKOVÁ (203 Czech Republic), Veronika HOLÁ (203 Czech Republic, belonging to the institution), Filip RŮŽIČKA (203 Czech Republic, belonging to the institution), Ladislav DUŠEK (203 Czech Republic, belonging to the institution), Petr NĚMEC (203 Czech Republic) and Tomáš FREIBERGER (203 Czech Republic, guarantor, belonging to the institution).
Edition European Journal of Clinical Microbiology & Infectious Diseases, 2012, 0934-9723.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 30201 Cardiac and Cardiovascular systems
Country of publisher United States of America
Confidentiality degree is not subject to a state or trade secret
Impact factor Impact factor: 3.024
RIV identification code RIV/00216224:14110/12:00059130
Organization unit Faculty of Medicine
Doi http://dx.doi.org/10.1007/s10096-011-1427-3
UT WoS 000303878400040
Keywords in English polymerase chain reaction; staphylococcal infective endocarditis
Tags International impact
Changed by Changed by: Soňa Böhmová, učo 232884. Changed: 22. 4. 2013 17:36.
Abstract
The aim of the study was to establish a diagnostic value for broad-range polymerase chain reaction (br-PCR) and staphylococci-specific multiplex PCR (ssm-PCR) performed on surgical material from patients with staphylococcal infective endocarditis (IE). Data were analysed retrospectively from 60 patients with suspected staphylococcal IE and 59 controls who were surgically treated at three cardiosurgery centres over 4 years. Both PCR tests showed high agreement and could be aggregated. In patients with definite and rejected IE, the clinical sensitivity and specificity of PCR reached 89 and 95%, respectively. Tissue culture (TC) and PCR agreed with blood culture (BC) in 29% and 67% of IE cases. TC helped to determine aetiology in five BC negative cases while PCR aided in nine cases. Out of 52 patients with conclusive staphylococcal IE, 40 were diagnosed with S. aureus and 12 with coagulasenegative staphylococci. PCR was shown to be highly superior to TC in confirming preoperative diagnosis of IE. In addition to aid in culture negative patients, PCR helped to establish or refine aetiology in inconclusive cases. We suggest that simultaneous br-PCR and ssm-PCR performed on surgical material together with histopathology could significantly increase the performance of current Duke criteria.
Links
2B08060, research and development projectName: Vývoj diagnostiky a léčby závažných onemocnění srdce a cév pomocí genomických a epigenomických přístupů (Acronym: EPICARD)
Investor: Ministry of Education, Youth and Sports of the CR, Development of diagnostics and treatment of serious heart and vascular diseases using genomic and proteomic approaches
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