a 2011

Specificity and affinity modulation of PA-IIL lectin

POKORNÁ, Martina, Jana MRÁZKOVÁ and Michaela WIMMEROVÁ

Basic information

Original name

Specificity and affinity modulation of PA-IIL lectin

Authors

POKORNÁ, Martina (203 Czech Republic, belonging to the institution), Jana MRÁZKOVÁ (203 Czech Republic, belonging to the institution) and Michaela WIMMEROVÁ (203 Czech Republic, guarantor, belonging to the institution)

Edition

36th FEBS Congress, 2011

Other information

Language

English

Type of outcome

Konferenční abstrakt

Field of Study

10600 1.6 Biological sciences

Country of publisher

Italy

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 3.790

RIV identification code

RIV/00216224:14740/11:00050179

Organization unit

Central European Institute of Technology

ISSN

UT WoS

000292333102532

Keywords in English

PA-IIL; PA-IL; Pseudomonas aeruginosa; mutagenesis

Tags

Změněno: 28/12/2011 13:24, Mgr. Martina Pokorná, Ph.D., MBA

Abstract

V originále

Pseudomonas aeruginosa is a clinically important pathogen, which is responsible for numerous nosocomial infections in immunocompromised patients. The bacterium colonises patients with chronic lung diseases and its infection is fatal in cystic fibrosis patients. P. aeruginosa produces high levels of D galactose and L-fucose binding lectins, PA-IL (LecA) and PA-IIL (LecB) respectively, which are associated with its cytotoxic virulence and could be involved in primary recognition of the host organism and in biofilm formation.Additional experiments have been performed to order to understand the molecular basis of both specificity and affinity of PA-IIL for monosaccharides. Three single point mutants in position 22-23-24 have prepared and structure-functioned characterized. The mutated amino acids belong to the “specificity-binding loop”. The in vitro mutagenesis in combination with computational methods allowed the key importance of amino acid 22 for the specificity of the lectin to be identified.

Links

GPP207/11/P185, research and development project
Name: Příprava lektinů s vysokou specifitou a afinitou
Investor: Czech Science Foundation