MATULA, Petr, Tautvydas LISAUSKAS, Vytaute STARKUVIENE and Karl ROHR. Quantification of Golgi Complex Assembly and Disassembly in LiveCell Fluorescence Microscopy Images. In Proceedings of Workshop on Microscopic Image Analysis with Applications in Biology. Neuveden: Neuveden, 2011. 4 pp.
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Basic information
Original name Quantification of Golgi Complex Assembly and Disassembly in LiveCell Fluorescence Microscopy Images
Authors MATULA, Petr, Tautvydas LISAUSKAS, Vytaute STARKUVIENE and Karl ROHR.
Edition Neuveden, Proceedings of Workshop on Microscopic Image Analysis with Applications in Biology, 4 pp. 2011.
Publisher Neuveden
Other information
Original language English
Type of outcome Proceedings paper
Field of Study 10201 Computer sciences, information science, bioinformatics
Country of publisher Germany
Confidentiality degree is not subject to a state or trade secret
WWW URL
Organization unit Faculty of Informatics
Keywords in English Fluorescence microscopy; quantification of subcellular localization patterns; tophat transform; Golgi assembly and disassembly; compact and diffuse phenotypes
Tags International impact, Reviewed
Changed by Changed by: doc. RNDr. Petr Matula, Ph.D., učo 3019. Changed: 17/4/2012 14:27.
Abstract
We present an image analysis approach for quantifying assembly and disassembly of the Golgi complex in fluorescence microscopy images of live cells. The approach comprises nucleus detection, nucleus tracking, and quantification of Golgi complex localization patterns for single cell nuclei over time. In particular, we define and compare five different measures for quantifying changes in compact and diffuse phenotypes. We propose two measures based on mathematical morphology which take into account shape, size, and contrast of image structures. From the experimental results it turned out that these measures have a higher discrimination power to distinguish compact and diffuse phenotypes than a previous frequently used measure.
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MSM0021622419, plan (intention)Name: Vysoce paralelní a distribuované výpočetní systémy
Investor: Ministry of Education, Youth and Sports of the CR, Research Intents
2B06052, research and development projectName: Vytipování markerů, screening a časná diagnostika nádorových onemocnění pomocí vysoce automatizovaného zpracování multidimenzionálních biomedicínských obrazů (Acronym: Biomarker)
Investor: Ministry of Education, Youth and Sports of the CR, Health and quality of life
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