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@inproceedings{959993,
author = {Matula, Petr and Lisauskas, Tautvydas and Starkuviene, Vytaute and Rohr, Karl},
address = {Neuveden},
booktitle = {Proceedings of Workshop on Microscopic Image Analysis with Applications in Biology},
keywords = {Fluorescence microscopy; quantification of subcellular localization patterns; tophat transform; Golgi assembly and disassembly; compact and diffuse phenotypes},
language = {eng},
location = {Neuveden},
publisher = {Neuveden},
title = {Quantification of Golgi Complex Assembly and Disassembly in LiveCell Fluorescence Microscopy Images},
url = {http://www.miaab.org/miaab-2011-heidelberg-papers/miaab-2011-h-matula.pdf},
year = {2011}
}
TY - JOUR
ID - 959993
AU - Matula, Petr - Lisauskas, Tautvydas - Starkuviene, Vytaute - Rohr, Karl
PY - 2011
TI - Quantification of Golgi Complex Assembly and Disassembly in LiveCell Fluorescence Microscopy Images
PB - Neuveden
CY - Neuveden
KW - Fluorescence microscopy
KW - quantification of subcellular localization patterns
KW - tophat transform
KW - Golgi assembly and disassembly
KW - compact and diffuse phenotypes
UR - http://www.miaab.org/miaab-2011-heidelberg-papers/miaab-2011-h-matula.pdf
L2 - http://www.miaab.org/miaab-2011-heidelberg-papers/miaab-2011-h-matula.pdf
N2 - We present an image analysis approach for quantifying assembly and disassembly of the Golgi complex in fluorescence microscopy images of live cells. The approach comprises nucleus detection, nucleus tracking, and quantification of Golgi complex localization patterns for single cell nuclei over time. In particular, we define and compare five different measures for quantifying changes in compact and diffuse phenotypes. We propose two measures based on mathematical morphology which take into account shape, size, and contrast of image structures. From the experimental results it turned out that these measures have a higher discrimination power to distinguish compact and diffuse phenotypes than a previous frequently used measure.
ER -
MATULA, Petr, Tautvydas LISAUSKAS, Vytaute STARKUVIENE and Karl ROHR. Quantification of Golgi Complex Assembly and Disassembly in LiveCell Fluorescence Microscopy Images. In \textit{Proceedings of Workshop on Microscopic Image Analysis with Applications in Biology}. Neuveden: Neuveden. 4 pp. 2011.
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