| LISAUSKAS, Tautvydas, Petr MATULA, Christoph CLAAS, Susanne REUSING, Stefan WIEMANN, Holger ERFLE, Lars LEHMANN, Peter FISCHER, Roland EILS, Karl ROHR, Brian STORRIE a Vytaute STARKUVIENE. Live cell assays to identify regulators of ER to Golgi trafficking. Traffic. John Wiley & Sons, roč. 13, č. 3, s. 416–432. ISSN 1398-9219. doi:10.1111/j.1600-0854.2011.01318.x. 2012. |
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@article{959996,
author = {Lisauskas, Tautvydas and Matula, Petr and Claas, Christoph and Reusing, Susanne and Wiemann, Stefan and Erfle, Holger and Lehmann, Lars and Fischer, Peter and Eils, Roland and Rohr, Karl and Storrie, Brian and Starkuviene, Vytaute},
article_number = {3},
doi = {http://dx.doi.org/10.1111/j.1600-0854.2011.01318.x},
keywords = {BFA; GalT; ER to Golgi trafficking; YIPF; GOT1B; USE1; SACM1L},
language = {eng},
issn = {1398-9219},
journal = {Traffic},
title = {Live cell assays to identify regulators of ER to Golgi trafficking},
volume = {13},
year = {2012}
}
TY - JOUR
ID - 959996
AU - Lisauskas, Tautvydas - Matula, Petr - Claas, Christoph - Reusing, Susanne - Wiemann, Stefan - Erfle, Holger - Lehmann, Lars - Fischer, Peter - Eils, Roland - Rohr, Karl - Storrie, Brian - Starkuviene, Vytaute
PY - 2012
TI - Live cell assays to identify regulators of ER to Golgi trafficking
JF - Traffic
VL - 13
IS - 3
SP - 416–432
EP - 416–432
PB - John Wiley & Sons
SN - 13989219
KW - BFA
KW - GalT
KW - ER to Golgi trafficking
KW - YIPF
KW - GOT1B
KW - USE1
KW - SACM1L
N2 - We used fluorescence microscopy based quantitative assays in living cells to identify regulators of ER to Golgi trafficking and/or Golgi complex maintenance. We first validated an automated procedure to identify factors, which influence Golgi to ER re-localization of GalT-CFP after BFA addition and wash-out. We then tested 14 proteins that potentially play a role in ER to Golgi trafficking, and localize to the ER and/or Golgi complex when over-expressed. 9 of them interfered with the rate of BFA induced redistribution of GalT-CFP to the ER, 6 of them interfered with GalT-CFP reassembly rate to a juxtanuclear region after BFA wash-out, and 6 of them were positive effectors in both assays. Notably, our live cell approach captures functions of those regulators of ER to Golgi trafficking, which were missed in previous fixed cell assays; as well as assigns respective roles for yet incompletely characterized proteins. Moreover, the assays can be extended to work under the conditions of RNAi and for testing chemical compounds.
ER -
LISAUSKAS, Tautvydas, Petr MATULA, Christoph CLAAS, Susanne REUSING, Stefan WIEMANN, Holger ERFLE, Lars LEHMANN, Peter FISCHER, Roland EILS, Karl ROHR, Brian STORRIE a Vytaute STARKUVIENE. Live cell assays to identify regulators of ER to Golgi trafficking. \textit{Traffic}. John Wiley \&{} Sons, roč.~13, č.~3, s.~416–432. ISSN~1398-9219. doi:10.1111/j.1600-0854.2011.01318.x. 2012.
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