ŽĎÁRSKÁ, Markéta, Pavlína ZATLOUKALOVÁ, David POTĚŠIL, Ondrej ŠEDO, Ondrej NOVAK, Bedrich PESEK, Eva MIKLÁNKOVÁ, Jana VAŠÍČKOVÁ, Zbyněk ZDRÁHAL and Jan HEJÁTKO. Specificity of the Arabidopsis Shoot and Root Response to Cytokinins. In 5th Central and Eastern European Proteomic Conference. 2011.
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Basic information
Original name Specificity of the Arabidopsis Shoot and Root Response to Cytokinins
Authors ŽĎÁRSKÁ, Markéta, Pavlína ZATLOUKALOVÁ, David POTĚŠIL, Ondrej ŠEDO, Ondrej NOVAK, Bedrich PESEK, Eva MIKLÁNKOVÁ, Jana VAŠÍČKOVÁ, Zbyněk ZDRÁHAL and Jan HEJÁTKO.
Edition 5th Central and Eastern European Proteomic Conference, 2011.
Other information
Original language English
Type of outcome Conference abstract
Field of Study Genetics and molecular biology
Country of publisher Czech Republic
Confidentiality degree is not subject to a state or trade secret
WWW 5th Central and Eastern European Proteomic Conference in Prague, Czech Republic
Organization unit Faculty of Science
Keywords in English Arabidopsis, cytokinin, ethylene, proteomic analysis, hormonal crosstalk
Tags International impact
Changed by Changed by: Mgr. Markéta Žďárská, Ph.D., učo 107631. Changed: 30/11/2011 13:11.
Abstract
Cytokinins (CK) have been implicated in regulation of many aspects of plant growth and development. Recent genetic studies suggest that CK play distinct and sometimes even opposite roles in the regulation of the shoot and root development in Arabidopsis. Here we present results of a comprehensive approach in the study of the differential shoot and root CK response. To analyze CK response six days old seedlings of Arabidopsis thaliana (Col-0) were treated with 5 uM CK N6-benzylaminopurine BAP and root and shoot tissues were analyzed separately on diverse levels. Interestingly we identified proteins associated with abscisic acid (ABA)-dependent regulations in shoots, while in roots we detected proteins linked with ethylene metabolism and signalling. To further substantiate the potential crosstalk of BAP with metabolism of other hormones, we have analyzed changes of the endogenous hormone levels after CK application. We observed strong tissue specificity caused by BAP application in endogenous levels of ABA and ACC, the rate-limiting precursor of the ethylene biosynthesis that corresponded to our results of the proteomic analysis.
Links
LC06034, research and development projectName: Regulace morfogeneze rostlinných buněk a orgánů
Investor: Ministry of Education, Youth and Sports of the CR, Regulation of morphogenesis of plant cells and organs
MSM0021622415, plan (intention)Name: Molekulární podstata buněčných a tkáňových regulací
Investor: Ministry of Education, Youth and Sports of the CR, Molecular basis of cell and tissue regulations
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