THE NUCLEAR PORE COMPLEXES CHANGED AFTER ULTRASOUND EXPOSURE

VAŠKOVICOVÁ, Naděžda, Roman JANISCH and Jiřina ŠKORPÍKOVÁ. THE NUCLEAR PORE COMPLEXES CHANGED AFTER ULTRASOUND EXPOSURE. 2011. ISBN 978-80-260-0849-1.

Basic information

• Original name: THE NUCLEAR PORE COMPLEXES CHANGED AFTER ULTRASOUND EXPOSURE
• Authors: VAŠKOVICOVÁ, Naděžda, Roman JANISCH and Jiřina ŠKORPÍKOVÁ.
• Edition: 2011.

Other information

• Original language: English
• Type of outcome: Conference abstract
• Field of Study: 10610 Biophysics
• Confidentiality degree: is not subject to a state or trade secret
• Organization unit: Faculty of Medicine
• ISBN: 978-80-260-0849-1

Abstract

Changes in the size of diameter of nuclear pore complexes fractured along a protoplasmic and an exoplasmic face of the inner and outer membrane of a nuclear envelope (freeze-etching method) in HL-60 cells after ultrasound exposure were studied. A cell suspension exposed at 1 MHz frequency and 1 W/cm2 intensity in a continuous mode for 10 min was placed in a 37 degree Celsius water bath; samples were collected at four different intervals (0 min, 10 min, 20 min, 30 min) and fixed in 2 % paraformaldehyde + 2.5 % glutaraldehyde in 0.1 M cacodylate buffer. The assessment showed significant differences in the size of diameter of nuclear pore complexes on the protoplasmic face between ultrasound-exposed and unexposed cells (P0=0.001, P10=0.000, P20=0.000). On the exoplasmic face there was a significant difference between ultrasound-exposed and unexposed cells only in the sample fixed immediately after sonication (P0=0.004). In the other samples the differences were not statistically significant. The study shows that therapeutic ultrasound has an effect on cellular structures formed from subunits and separate proteins whose bonds can be disintegrated by mechanical waves. The visual manifestation of it is an increase in the size of diameter of nuclear pore complexes on fractured membrane faces.