Detailed Information on Publication Record
2011
VISUALISATION OF A PROTEIN DISTRIBUTION BY USING THE FREEZE-ETCHING METHOD
VAŠKOVICOVÁ, Naděžda, Andrea VALIGUROVÁ and Roman JANISCHBasic information
Original name
VISUALISATION OF A PROTEIN DISTRIBUTION BY USING THE FREEZE-ETCHING METHOD
Name in Czech
VISUALISATION OF A PROTEIN DISTRIBUTION BY USING THE FREEZE-ETCHING METHOD
Authors
VAŠKOVICOVÁ, Naděžda (203 Czech Republic, guarantor, belonging to the institution), Andrea VALIGUROVÁ (703 Slovakia, belonging to the institution) and Roman JANISCH (203 Czech Republic, belonging to the institution)
Edition
2011
Other information
Language
English
Type of outcome
Konferenční abstrakt
Field of Study
10610 Biophysics
Country of publisher
Czech Republic
Confidentiality degree
není předmětem státního či obchodního tajemství
RIV identification code
RIV/00216224:14110/11:00057214
Organization unit
Faculty of Medicine
ISBN
978-80-263-0050-2
Keywords (in Czech)
freeze etching; membranes; gregarines
Keywords in English
freeze etching; membranes; gregarines
Změněno: 20/1/2013 12:02, doc. RNDr. Andrea Bardůnek Valigurová, Ph.D.
V originále
The freeze-etching is one of cryo-methods, which is used to visualize the membrane structure of the cells. In general, this method provides information about the protrusions of the cell membrane or the undulation of surface of the plasma membrane, but by the processing of samples it is very common to achieve fracturing through the organelles inside the cells, i.e. mitochondria, endoplasmic reticulum or nuclear envelope with nuclear pores. As the membranes are usually fractured on their protoplasmic or exoplasmic face, it is possible to observe the trans-membrane or intra-membrane proteins without the need of specific labelling. The replica of membranes is 25 nm thick, and is formed by a thin layer of platinum and carbon. The distribution of proteins on the membrane, changes of the proteins concentration caused by various physical or chemical factors, number of nuclear pores in the nuclear envelope, and changes in their distribution due to cell cycle or apoptosis can be quantitatively evaluated after an image analysis.
In Czech
The freeze-etching is one of cryo-methods, which is used to visualize the membrane structure of the cells. In general, this method provides information about the protrusions of the cell membrane or the undulation of surface of the plasma membrane, but by the processing of samples it is very common to achieve fracturing through the organelles inside the cells, i.e. mitochondria, endoplasmic reticulum or nuclear envelope with nuclear pores. As the membranes are usually fractured on their protoplasmic or exoplasmic face, it is possible to observe the trans-membrane or intra-membrane proteins without the need of specific labelling. The replica of membranes is 25 nm thick, and is formed by a thin layer of platinum and carbon. The distribution of proteins on the membrane, changes of the proteins concentration caused by various physical or chemical factors, number of nuclear pores in the nuclear envelope, and changes in their distribution due to cell cycle or apoptosis can be quantitatively evaluated after an image analysis.
Links
| GPP506/10/P372, research and development project |
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