VISUALISATION OF A PROTEIN DISTRIBUTION BY USING THE FREEZE-ETCHING METHOD

VAŠKOVICOVÁ, Naděžda, Andrea VALIGUROVÁ and Roman JANISCH. VISUALISATION OF A PROTEIN DISTRIBUTION BY USING THE FREEZE-ETCHING METHOD. 2011. ISBN 978-80-263-0050-2.

Basic information

• Original name: VISUALISATION OF A PROTEIN DISTRIBUTION BY USING THE FREEZE-ETCHING METHOD
• Name in Czech: VISUALISATION OF A PROTEIN DISTRIBUTION BY USING THE FREEZE-ETCHING METHOD
• Authors: VAŠKOVICOVÁ, Naděžda (203 Czech Republic, guarantor, belonging to the institution), Andrea VALIGUROVÁ (703 Slovakia, belonging to the institution) and Roman JANISCH (203 Czech Republic, belonging to the institution).
• Edition: 2011.

Other information

• Original language: English
• Type of outcome: Conference abstract
• Field of Study: 10610 Biophysics
• Country of publisher: Czech Republic
• Confidentiality degree: is not subject to a state or trade secret
• RIV identification code: RIV/00216224:14110/11:00057214
• Organization unit: Faculty of Medicine
• ISBN: 978-80-263-0050-2
• Keywords (in Czech): freeze etching; membranes; gregarines
• Keywords in English: freeze etching; membranes; gregarines

Abstract

The freeze-etching is one of cryo-methods, which is used to visualize the membrane structure of the cells. In general, this method provides information about the protrusions of the cell membrane or the undulation of surface of the plasma membrane, but by the processing of samples it is very common to achieve fracturing through the organelles inside the cells, i.e. mitochondria, endoplasmic reticulum or nuclear envelope with nuclear pores. As the membranes are usually fractured on their protoplasmic or exoplasmic face, it is possible to observe the trans-membrane or intra-membrane proteins without the need of specific labelling. The replica of membranes is 25 nm thick, and is formed by a thin layer of platinum and carbon. The distribution of proteins on the membrane, changes of the proteins concentration caused by various physical or chemical factors, number of nuclear pores in the nuclear envelope, and changes in their distribution due to cell cycle or apoptosis can be quantitatively evaluated after an image analysis.

Abstract (in Czech)

The freeze-etching is one of cryo-methods, which is used to visualize the membrane structure of the cells. In general, this method provides information about the protrusions of the cell membrane or the undulation of surface of the plasma membrane, but by the processing of samples it is very common to achieve fracturing through the organelles inside the cells, i.e. mitochondria, endoplasmic reticulum or nuclear envelope with nuclear pores. As the membranes are usually fractured on their protoplasmic or exoplasmic face, it is possible to observe the trans-membrane or intra-membrane proteins without the need of specific labelling. The replica of membranes is 25 nm thick, and is formed by a thin layer of platinum and carbon. The distribution of proteins on the membrane, changes of the proteins concentration caused by various physical or chemical factors, number of nuclear pores in the nuclear envelope, and changes in their distribution due to cell cycle or apoptosis can be quantitatively evaluated after an image analysis.

Links

• GPP506/10/P372, research and development project: Name: Srovnávací morfologie a imunohistochemie v hodnocení fylogenetických vztahů mezi zástupci raných linií kmene Apicomplexa

Investor: Czech Science Foundation