Experimental Administration of the Probiotic Escherichia coli
Strain Nissle 1917 Results in Decreased Diversity of E. coli
Strains in Pigs

J 2012

Experimental Administration of the Probiotic Escherichia coli Strain Nissle 1917 Results in Decreased Diversity of E. coli Strains in Pigs

ŠMAJS, David, Jan BUREŠ, Jan ŠMARDA, Eva CHALOUPKOVÁ, Jaroslav KVĚTINA et. al.

Basic information

Original name

Experimental Administration of the Probiotic Escherichia coli Strain Nissle 1917 Results in Decreased Diversity of E. coli Strains in Pigs

Authors

ŠMAJS, David (203 Czech Republic, guarantor, belonging to the institution), Jan BUREŠ (203 Czech Republic), Jan ŠMARDA (203 Czech Republic, belonging to the institution), Eva CHALOUPKOVÁ (203 Czech Republic, belonging to the institution), Jaroslav KVĚTINA (203 Czech Republic), Miroslav FÖRSTL (203 Czech Republic), Darina KOHOUTOVÁ (203 Czech Republic), Martin KUNEŠ (203 Czech Republic), Stanislav REJCHRT (203 Czech Republic), Jiřina LESNÁ (203 Czech Republic) and Marcela KOPÁČOVÁ (203 Czech Republic)

Edition

Current Microbiology, 2012, 0343-8651

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 1.520

RIV identification code

RIV/00216224:14110/12:00059148

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1007/s00284-011-0051-x

UT WoS

000300769800001

Keywords in English

Escherichia coli; pig; strain Escherichia coli Nissle 1917

Abstract

V originále

The strain Escherichia coli Nissle 1917 (EcN) is widely used as an efficient probiotic in therapy and prevention of human infectious diseases, especially of the intestinal system. Concurrently, small adult pigs are being used as experimental omnivore models to study human gastrointestinal functions. EcN bacteria were applied to 6 adult healthy female pigs in a 2-week trial. 6 Control animals remained untreated. Altogether, 164 and 149 bacterial strains were isolated from smear samples taken from gastrointestinal mucosa in the experimental and control group, respectively. Each individual E. coli strain was then tested for the presence of 29 bacteriocin-encoding determinants as well as for DNA markers of A, B1, B2 and D phylogenetic groups. A profound reduction of E. coli genetic variance (from 32 variants to 13 ones, P = 0.0006) was found in the experimental group, accompanied by a lower incidence of bacteriocin producers in the experimental group when compared to control (21.3 and 34.9%, respectively; P = 0.007) and by changes in the incidence of individual bacteriocin types. The experimental administration of EcN strain was not sufficient for stable colonization of porcine gut, but induced significant changes in the enterobacterial microbiota.

Links

MSM0021622415, plan (intention)

Name: Molekulární podstata buněčných a tkáňových regulací

Investor: Ministry of Education, Youth and Sports of the CR, Molecular basis of cell and tissue regulations

NS9665, research and development project

Name: Molekulární typizace bakteriocinů produkovaných kmeny Escherichia coli z klinického materiálu: identifikace kolicinů a mikrocinů v souborech komensálních a patogenních kmenů

Investor: Ministry of Health of the CR

Citovat

ŠMAJS, David, Jan BUREŠ, Jan ŠMARDA, Eva CHALOUPKOVÁ, Jaroslav KVĚTINA, Miroslav FÖRSTL, Darina KOHOUTOVÁ, Martin KUNEŠ, Stanislav REJCHRT, Jiřina LESNÁ and Marcela KOPÁČOVÁ. Experimental Administration of the Probiotic Escherichia coli Strain Nissle 1917 Results in Decreased Diversity of E. coli Strains in Pigs. Current Microbiology. 2012, vol. 64, No 3, p. 205-210. ISSN 0343-8651. Available from: https://dx.doi.org/10.1007/s00284-011-0051-x.

@article{961061,
   author = {Šmajs, David and Bureš, Jan and Šmarda, Jan and Chaloupková, Eva and Květina, Jaroslav and Förstl, Miroslav and Kohoutová, Darina and Kuneš, Martin and Rejchrt, Stanislav and Lesná, Jiřina and Kopáčová, Marcela},
   article_number = {3},
   doi = {http://dx.doi.org/10.1007/s00284-011-0051-x},
   keywords = {Escherichia coli; pig; strain Escherichia coli Nissle 1917},
   language = {eng},
   issn = {0343-8651},
   journal = {Current Microbiology},
   title = {Experimental Administration of the Probiotic Escherichia coli Strain Nissle 1917 Results in Decreased Diversity of E. coli Strains in Pigs},
   volume = {64},
   year = {2012}
}

TY  - JOUR
ID  - 961061
AU  - Šmajs, David - Bureš, Jan - Šmarda, Jan - Chaloupková, Eva - Květina, Jaroslav - Förstl, Miroslav - Kohoutová, Darina - Kuneš, Martin - Rejchrt, Stanislav - Lesná, Jiřina - Kopáčová, Marcela
PY  - 2012
TI  - Experimental Administration of the Probiotic Escherichia coli Strain Nissle 1917 Results in Decreased Diversity of E. coli Strains in Pigs
JF  - Current Microbiology
VL  - 64
IS  - 3
SP  - 205-210
EP  - 205-210
SN  - 03438651
KW  - Escherichia coli
KW  - pig
KW  - strain Escherichia coli Nissle 1917
N2  - The strain Escherichia coli Nissle 1917 (EcN) is widely used as an efficient probiotic in therapy and prevention of human infectious diseases, especially of the intestinal system. Concurrently, small adult pigs are being used as experimental omnivore models to study human gastrointestinal functions. EcN bacteria were applied to 6 adult healthy female pigs in a 2-week trial. 6 Control animals remained untreated. Altogether, 164 and 149 bacterial strains were isolated from smear samples taken from gastrointestinal mucosa in the experimental and control group, respectively. Each individual E. coli strain was then tested for the presence of 29 bacteriocin-encoding determinants as well as for DNA markers of A, B1, B2 and D phylogenetic groups. A profound reduction of E. coli genetic variance (from 32 variants to 13 ones, P = 0.0006) was found in the experimental group, accompanied by a lower incidence of bacteriocin producers in the experimental group when compared to control (21.3 and 34.9%, respectively; P = 0.007) and by changes in the incidence of individual bacteriocin types. The experimental administration of EcN strain was not sufficient for stable colonization of porcine gut, but induced significant changes in the enterobacterial microbiota.
ER  -

ŠMAJS, David, Jan BUREŠ, Jan ŠMARDA, Eva CHALOUPKOVÁ, Jaroslav KVĚTINA, Miroslav FÖRSTL, Darina KOHOUTOVÁ, Martin KUNEŠ, Stanislav REJCHRT, Jiřina LESNÁ and Marcela KOPÁČOVÁ. Experimental Administration of the Probiotic Escherichia coli Strain Nissle 1917 Results in Decreased Diversity of E. coli Strains in Pigs. \textit{Current Microbiology}. 2012, vol.~64, No~3, p.~205-210. ISSN~0343-8651. Available from: https://dx.doi.org/10.1007/s00284-011-0051-x.

Detailed Information on Publication Record