Phosphoinositide 3-kinase inhibition enables retinoic acid-induced neurogenesis in monolayer culture of embryonic stem cells.

KOTASOVÁ, Hana, Iva VESELÁ, Jan KUČERA, Zbyněk HOUDEK, Jiřina PROCHÁZKOVÁ, Milena KRÁLÍČKOVÁ and Jiří PACHERNÍK. Phosphoinositide 3-kinase inhibition enables retinoic acid-induced neurogenesis in monolayer culture of embryonic stem cells. J Cell Biochem. Wiley-Liss, Inc., 2012, vol. 113, No 2, p. 563-570. ISSN 0730-2312. Available from: https://dx.doi.org/10.1002/jcb.23380.

Basic information

• Original name: Phosphoinositide 3-kinase inhibition enables retinoic acid-induced neurogenesis in monolayer culture of embryonic stem cells.
• Authors: KOTASOVÁ, Hana (203 Czech Republic, guarantor, belonging to the institution), Iva VESELÁ (203 Czech Republic, belonging to the institution), Jan KUČERA (203 Czech Republic, belonging to the institution), Zbyněk HOUDEK (203 Czech Republic), Jiřina PROCHÁZKOVÁ (203 Czech Republic, belonging to the institution), Milena KRÁLÍČKOVÁ (203 Czech Republic) and Jiří PACHERNÍK (203 Czech Republic, belonging to the institution).
• Edition: J Cell Biochem, Wiley-Liss, Inc. 2012, 0730-2312.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: 30105 Physiology
• Country of publisher: United States of America
• Confidentiality degree: is not subject to a state or trade secret
• Impact factor: Impact factor: 3.062
• RIV identification code: RIV/00216224:14310/12:00057219
• Organization unit: Faculty of Science
• Doi: http://dx.doi.org/10.1002/jcb.23380
• UT WoS: 000298843200022
• Keywords in English: Embryonic stem cell;retionic acid;neurogenesis;differentiation
• Tags: AKR, rivok
• Tags: International impact, Reviewed

Abstract

Retinoic acid is able to induce the differentiation of embryonic stem cells into neuronal lineages. The mechanism of this effect is unknown but it has been evidenced to be dependent on the formation of floating spheroids called embryoid bodies. Results presented here show that the inhibition of phosphoinositide 3-kinase signaling predetermines mouse embryonic stem cells to retinoic acid induced neurogenesis in monolayer culture with no need of embryoid bodies formation.

Links

• GA301/08/0717, research and development project: Name: Regulace diferenciace ES buněk prostřednictvím receptoru gp130

Investor: Czech Science Foundation, Gp130 receptor-mediated differentiation of ES cells

• GD204/09/H058, research and development project: Name: Mezibuněčná signalizace ve vývoji organismu a vzniku onemocnění

Investor: Czech Science Foundation, Intercellular signalling in development and disease

• MSM0021622430, plan (intention): Name: Funkční a molekulární charakteristiky nádorových a normálních kmenových buněk - identifikace cílů pro nová terapeutika a terapeutické strategie

Investor: Ministry of Education, Youth and Sports of the CR, Functional and molecular characteristics of cancer and normal stem cells - identification of targets for novel therapeutics and therapeutic strategies