ABC transporters affect the detection of intracellular oxidants
by fluorescent probes.

J 2011

ABC transporters affect the detection of intracellular oxidants by fluorescent probes.

PROCHÁZKOVÁ, Jiřina, Lukáš KUBALA, Hana KOTASOVÁ, Iva GUDERNOVÁ, Zuzana ŠRÁMKOVÁ et. al.

Basic information

Original name

ABC transporters affect the detection of intracellular oxidants by fluorescent probes.

Authors

PROCHÁZKOVÁ, Jiřina (203 Czech Republic, guarantor, belonging to the institution), Lukáš KUBALA (203 Czech Republic, belonging to the institution), Hana KOTASOVÁ (203 Czech Republic, belonging to the institution), Iva GUDERNOVÁ (203 Czech Republic, belonging to the institution), Zuzana ŠRÁMKOVÁ (703 Slovakia, belonging to the institution), Michaela PEKAROVÁ (703 Slovakia, belonging to the institution), Balazs SARKADI (348 Hungary) and Jiří PACHERNÍK (203 Czech Republic, belonging to the institution)

Edition

Free Radical Res. 2011, 1071-5762

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30105 Physiology

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 2.878

RIV identification code

RIV/00216224:14310/11:00050241

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.3109/10715762.2011.579120

UT WoS

000291222100004

Keywords in English

Fluorescence; redox status; mitochondria; reactive oxygen species (ROS)

Abstract

V originále

Intracellular production of reactive oxygen species (ROS) plays an important role in the control of cell physiology. For the assessment of intracellular ROS production, a plethora of fluorescent probes is commonly used. Interestingly, chemical structures of these probes imply they could be substrates of plasma membrane efflux pumps, called ABC transporters. This study tested whether the determination of intracellular ROS production and mitochondrial membrane potential by selected fluorescent probes is modulated by the expression and activity of ABC transporters.

Links

GA301/08/0717, research and development project

Name: Regulace diferenciace ES buněk prostřednictvím receptoru gp130

Investor: Czech Science Foundation, Gp130 receptor-mediated differentiation of ES cells

Citovat

PROCHÁZKOVÁ, Jiřina, Lukáš KUBALA, Hana KOTASOVÁ, Iva GUDERNOVÁ, Zuzana ŠRÁMKOVÁ, Michaela PEKAROVÁ, Balazs SARKADI and Jiří PACHERNÍK. ABC transporters affect the detection of intracellular oxidants by fluorescent probes. Free Radical Res. 2011, vol. 45, No 7, p. 779-787. ISSN 1071-5762. Available from: https://dx.doi.org/10.3109/10715762.2011.579120.

@article{962368,
   author = {Procházková, Jiřina and Kubala, Lukáš and Kotasová, Hana and Gudernová, Iva and Šrámková, Zuzana and Pekarová, Michaela and Sarkadi, Balazs and Pacherník, Jiří},
   article_number = {7},
   doi = {http://dx.doi.org/10.3109/10715762.2011.579120},
   keywords = {Fluorescence; redox status; mitochondria; reactive oxygen species (ROS)},
   language = {eng},
   issn = {1071-5762},
   journal = {Free Radical Res.},
   title = {ABC transporters affect the detection of intracellular oxidants by fluorescent probes.},
   volume = {45},
   year = {2011}
}

TY  - JOUR
ID  - 962368
AU  - Procházková, Jiřina - Kubala, Lukáš - Kotasová, Hana - Gudernová, Iva - Šrámková, Zuzana - Pekarová, Michaela - Sarkadi, Balazs - Pacherník, Jiří
PY  - 2011
TI  - ABC transporters affect the detection of intracellular oxidants by fluorescent probes.
JF  - Free Radical Res.
VL  - 45
IS  - 7
SP  - 779-787
EP  - 779-787
SN  - 10715762
KW  - Fluorescence
KW  - redox status
KW  - mitochondria
KW  - reactive oxygen species (ROS)
N2  - Intracellular production of reactive oxygen species (ROS) plays an important role in the control of cell physiology. For the assessment of intracellular ROS production, a plethora of fluorescent probes is commonly used. Interestingly, chemical structures of these probes imply they could be substrates of plasma membrane efflux pumps, called ABC transporters. This study tested whether the determination of intracellular ROS production and mitochondrial membrane potential by selected fluorescent probes is modulated by the expression and activity of ABC transporters.
ER  -

PROCHÁZKOVÁ, Jiřina, Lukáš KUBALA, Hana KOTASOVÁ, Iva GUDERNOVÁ, Zuzana ŠRÁMKOVÁ, Michaela PEKAROVÁ, Balazs SARKADI and Jiří PACHERNÍK. ABC transporters affect the detection of intracellular oxidants by fluorescent probes. \textit{Free Radical Res.}. 2011, vol.~45, No~7, p.~779-787. ISSN~1071-5762. Available from: https://dx.doi.org/10.3109/10715762.2011.579120.

Detailed Information on Publication Record