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@article{962577,
author = {Majerová, Eva and Fojtová, Miloslava and Mozgová, Iva and Bittová, Miroslava and Fajkus, Jiří},
article_number = {4-5},
doi = {http://dx.doi.org/10.1007/s11103-011-9816-7},
keywords = {Nicotiana tabacum; Cell culture; Telomere; Hypomethylating agents; Zebularine; DNA methylation},
language = {eng},
issn = {0167-4412},
journal = {Plant Molecular Biology},
title = {Hypomethylating drugs efficiently decrease cytosine methylationin telomeric DNA and activate telomerase without affectingtelomere lengths in tobacco cells},
volume = {77},
year = {2011}
}
TY - JOUR
ID - 962577
AU - Majerová, Eva - Fojtová, Miloslava - Mozgová, Iva - Bittová, Miroslava - Fajkus, Jiří
PY - 2011
TI - Hypomethylating drugs efficiently decrease cytosine methylationin telomeric DNA and activate telomerase without affectingtelomere lengths in tobacco cells
JF - Plant Molecular Biology
VL - 77
IS - 4-5
SP - 371-380
EP - 371-380
PB - Springer
SN - 01674412
KW - Nicotiana tabacum
KW - Cell culture
KW - Telomere
KW - Hypomethylating agents
KW - Zebularine
KW - DNA methylation
N2 - Telomere homeostasis is regulated at multiple levels, including the local chromatin structure of telomeres and subtelomeres. Recent reports demonstrated that a decrease in repressive chromatin marks, such as levels of cytosine methylation in subtelomeric regions, results in telomere elongation in mouse cells. Here we show that a considerable fraction of cytosines is methylated not only in subtelomeric, but also in telomeric DNA of tobacco BY-2 cells. Drug-induced hypomethylation (demonstrated at subtelomeric, telomeric, and global DNA levels) results in activation of telomerase. However, in contrast to mouse cells, the decrease in 5-methylcytosine levels and upregulation of telomerase do not result in any changes of telomere lengths. These results demonstrate the involvement of epigenetic mechanisms in the multilevel process of regulation of telomerase activity in plant cells and, at the same time, they indicate that changes in telomerase activity can be overridden by other factors governing telomere length stability.
ER -
MAJEROVÁ, Eva, Miloslava FOJTOVÁ, Iva MOZGOVÁ, Miroslava BITTOVÁ a Jiří FAJKUS. Hypomethylating drugs efficiently decrease cytosine methylationin telomeric DNA and activate telomerase without affectingtelomere lengths in tobacco cells. \textit{Plant Molecular Biology}. Springer, 2011, roč.~77, 4-5, s.~371-380. ISSN~0167-4412. Dostupné z: https://dx.doi.org/10.1007/s11103-011-9816-7.
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