Other formats:
BibTeX
LaTeX
RIS
@article{962577, author = {Majerová, Eva and Fojtová, Miloslava and Mozgová, Iva and Bittová, Miroslava and Fajkus, Jiří}, article_number = {4-5}, doi = {http://dx.doi.org/10.1007/s11103-011-9816-7}, keywords = {Nicotiana tabacum; Cell culture; Telomere; Hypomethylating agents; Zebularine; DNA methylation}, language = {eng}, issn = {0167-4412}, journal = {Plant Molecular Biology}, title = {Hypomethylating drugs efficiently decrease cytosine methylationin telomeric DNA and activate telomerase without affectingtelomere lengths in tobacco cells}, volume = {77}, year = {2011} }
TY - JOUR ID - 962577 AU - Majerová, Eva - Fojtová, Miloslava - Mozgová, Iva - Bittová, Miroslava - Fajkus, Jiří PY - 2011 TI - Hypomethylating drugs efficiently decrease cytosine methylationin telomeric DNA and activate telomerase without affectingtelomere lengths in tobacco cells JF - Plant Molecular Biology VL - 77 IS - 4-5 SP - 371-380 EP - 371-380 PB - Springer SN - 01674412 KW - Nicotiana tabacum KW - Cell culture KW - Telomere KW - Hypomethylating agents KW - Zebularine KW - DNA methylation N2 - Telomere homeostasis is regulated at multiple levels, including the local chromatin structure of telomeres and subtelomeres. Recent reports demonstrated that a decrease in repressive chromatin marks, such as levels of cytosine methylation in subtelomeric regions, results in telomere elongation in mouse cells. Here we show that a considerable fraction of cytosines is methylated not only in subtelomeric, but also in telomeric DNA of tobacco BY-2 cells. Drug-induced hypomethylation (demonstrated at subtelomeric, telomeric, and global DNA levels) results in activation of telomerase. However, in contrast to mouse cells, the decrease in 5-methylcytosine levels and upregulation of telomerase do not result in any changes of telomere lengths. These results demonstrate the involvement of epigenetic mechanisms in the multilevel process of regulation of telomerase activity in plant cells and, at the same time, they indicate that changes in telomerase activity can be overridden by other factors governing telomere length stability. ER -
MAJEROVÁ, Eva, Miloslava FOJTOVÁ, Iva MOZGOVÁ, Miroslava BITTOVÁ and Jiří FAJKUS. Hypomethylating drugs efficiently decrease cytosine methylationin telomeric DNA and activate telomerase without affectingtelomere lengths in tobacco cells. \textit{Plant Molecular Biology}. Springer, 2011, vol.~77, 4-5, p.~371-380. ISSN~0167-4412. Available from: https://dx.doi.org/10.1007/s11103-011-9816-7.
|