Detailed Information on Publication Record
2011
Hypomethylating drugs efficiently decrease cytosine methylationin telomeric DNA and activate telomerase without affectingtelomere lengths in tobacco cells
MAJEROVÁ, Eva, Miloslava FOJTOVÁ, Iva MOZGOVÁ, Miroslava BITTOVÁ, Jiří FAJKUS et. al.Basic information
Original name
Hypomethylating drugs efficiently decrease cytosine methylationin telomeric DNA and activate telomerase without affectingtelomere lengths in tobacco cells
Authors
MAJEROVÁ, Eva (203 Czech Republic, belonging to the institution), Miloslava FOJTOVÁ (203 Czech Republic, belonging to the institution), Iva MOZGOVÁ (203 Czech Republic, belonging to the institution), Miroslava BITTOVÁ (203 Czech Republic, belonging to the institution) and Jiří FAJKUS (203 Czech Republic, guarantor, belonging to the institution)
Edition
Plant Molecular Biology, Springer, 2011, 0167-4412
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
Genetics and molecular biology
Country of publisher
Netherlands
Confidentiality degree
není předmětem státního či obchodního tajemství
Impact factor
Impact factor: 4.150
RIV identification code
RIV/00216224:14740/11:00050252
Organization unit
Central European Institute of Technology
UT WoS
000299051200004
Keywords in English
Nicotiana tabacum; Cell culture; Telomere; Hypomethylating agents; Zebularine; DNA methylation
Tags
International impact, Reviewed
Změněno: 29/3/2012 23:31, Olga Křížová
Abstract
V originále
Telomere homeostasis is regulated at multiple levels, including the local chromatin structure of telomeres and subtelomeres. Recent reports demonstrated that a decrease in repressive chromatin marks, such as levels of cytosine methylation in subtelomeric regions, results in telomere elongation in mouse cells. Here we show that a considerable fraction of cytosines is methylated not only in subtelomeric, but also in telomeric DNA of tobacco BY-2 cells. Drug-induced hypomethylation (demonstrated at subtelomeric, telomeric, and global DNA levels) results in activation of telomerase. However, in contrast to mouse cells, the decrease in 5-methylcytosine levels and upregulation of telomerase do not result in any changes of telomere lengths. These results demonstrate the involvement of epigenetic mechanisms in the multilevel process of regulation of telomerase activity in plant cells and, at the same time, they indicate that changes in telomerase activity can be overridden by other factors governing telomere length stability.
Links
| ED1.1.00/02.0068, research and development project |
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| GAP501/11/0289, research and development project |
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| GAP501/11/0596, research and development project |
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| LC06035, research and development project |
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| MSM0021622415, plan (intention) |
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