Hypomethylating drugs efficiently decrease cytosine methylationin telomeric DNA and activate telomerase without affectingtelomere lengths in tobacco cells

MAJEROVÁ, Eva, Miloslava FOJTOVÁ, Iva MOZGOVÁ, Miroslava BITTOVÁ and Jiří FAJKUS. Hypomethylating drugs efficiently decrease cytosine methylationin telomeric DNA and activate telomerase without affectingtelomere lengths in tobacco cells. Plant Molecular Biology. Springer, vol. 77, 4-5, p. 371-380. ISSN 0167-4412. doi:10.1007/s11103-011-9816-7. 2011.

Basic information

• Original name: Hypomethylating drugs efficiently decrease cytosine methylationin telomeric DNA and activate telomerase without affectingtelomere lengths in tobacco cells
• Authors: MAJEROVÁ, Eva (203 Czech Republic, belonging to the institution), Miloslava FOJTOVÁ (203 Czech Republic, belonging to the institution), Iva MOZGOVÁ (203 Czech Republic, belonging to the institution), Miroslava BITTOVÁ (203 Czech Republic, belonging to the institution) and Jiří FAJKUS (203 Czech Republic, guarantor, belonging to the institution).
• Edition: Plant Molecular Biology, Springer, 2011, 0167-4412.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: Genetics and molecular biology
• Country of publisher: Netherlands
• Confidentiality degree: is not subject to a state or trade secret
• Impact factor: Impact factor: 4.150
• RIV identification code: RIV/00216224:14740/11:00050252
• Organization unit: Central European Institute of Technology
• Doi: http://dx.doi.org/10.1007/s11103-011-9816-7
• UT WoS: 000299051200004
• Keywords in English: Nicotiana tabacum; Cell culture; Telomere; Hypomethylating agents; Zebularine; DNA methylation
• Tags: ok, rivok
• Tags: International impact, Reviewed

Abstract

Telomere homeostasis is regulated at multiple levels, including the local chromatin structure of telomeres and subtelomeres. Recent reports demonstrated that a decrease in repressive chromatin marks, such as levels of cytosine methylation in subtelomeric regions, results in telomere elongation in mouse cells. Here we show that a considerable fraction of cytosines is methylated not only in subtelomeric, but also in telomeric DNA of tobacco BY-2 cells. Drug-induced hypomethylation (demonstrated at subtelomeric, telomeric, and global DNA levels) results in activation of telomerase. However, in contrast to mouse cells, the decrease in 5-methylcytosine levels and upregulation of telomerase do not result in any changes of telomere lengths. These results demonstrate the involvement of epigenetic mechanisms in the multilevel process of regulation of telomerase activity in plant cells and, at the same time, they indicate that changes in telomerase activity can be overridden by other factors governing telomere length stability.

Links

• ED1.1.00/02.0068, research and development project: Name: CEITEC - central european institute of technology
• GAP501/11/0289, research and development project: Name: Ztráta specifických repetic DNA v důsledku dysfunkce CAF1 u rostlin

Investor: Czech Science Foundation

• GAP501/11/0596, research and development project: Name: Epigenetické mechanismy regulace rostlinných telomer (Acronym: epigenetika telomer)

Investor: Czech Science Foundation

• LC06035, research and development project: Name: Centrum biofyzikální chemie, bioelektrochemie a bioanalýzy. Nové nástroje pro genomiku, proteomiku a biomedicínu.

Investor: Ministry of Education, Youth and Sports of the CR, Centre of Biophysical Chemistry, Bioelectrochemistry and Bioanalysis. New Tools for Genomics, Proteomics and Biomedicine

• MSM0021622415, plan (intention): Name: Molekulární podstata buněčných a tkáňových regulací

Investor: Ministry of Education, Youth and Sports of the CR, Molecular basis of cell and tissue regulations