Methylation of plant telomeric DNA: what do the results say?

MAJEROVÁ, Eva, Miloslava FOJTOVÁ, Terezie MANDÁKOVÁ and Jiří FAJKUS. Methylation of plant telomeric DNA: what do the results say? Plant Molecular Biology. Springer, 2011, vol. 77, No 6, p. 533-536. ISSN 0167-4412. Available from: https://dx.doi.org/10.1007/s11103-011-9834-5.

Basic information

• Original name: Methylation of plant telomeric DNA: what do the results say?
• Authors: MAJEROVÁ, Eva (203 Czech Republic, belonging to the institution), Miloslava FOJTOVÁ (203 Czech Republic, belonging to the institution), Terezie MANDÁKOVÁ (203 Czech Republic, belonging to the institution) and Jiří FAJKUS (203 Czech Republic, guarantor, belonging to the institution).
• Edition: Plant Molecular Biology, Springer, 2011, 0167-4412.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: Genetics and molecular biology
• Country of publisher: Netherlands
• Confidentiality degree: is not subject to a state or trade secret
• Impact factor: Impact factor: 4.150
• RIV identification code: RIV/00216224:14740/11:00049500
• Organization unit: Central European Institute of Technology
• Doi: http://dx.doi.org/10.1007/s11103-011-9834-5
• UT WoS: 000299051300002
• Keywords in English: Nicotiana tabacum; Cell culture Telomere; Hypomethylating agents; Zebularine; DNA methylation
• Tags: ok, rivok
• Tags: International impact, Reviewed

Abstract

Recently, we reported (Majerova et al. 2011) that hypomethylating drugs efficiently decrease methylation of telomeric DNA and activate telomerase without affecting telomere lengths in tobacco. The key issue of the Letter to Editor written by Vaquero-Sedas and Vega-Palas (2011) is, whether our results demonstrating methylation and druginduced hypomethylation of tobacco telomeric DNA repeats do correspond to the status of terminal telomeric sequences, or rather to internal telomeric sequences (ITSs) which might exist in tobacco genome as it is the case in Arabidopsis thaliana, the model organism which Vaquero- Sedas and Vega-Palas have been working with. They are referring to their recent article (Vaquero-Sedas et al. 2010) in which they conclude that ITSs in A. thaliana are heterochromatic, while telomeres are euchromatic. We are pleased that our paper has initiated this debate and take the opportunity to go through the key points of the Letter of Vaquero-Sedas and Vega-Palas

Links

• GA204/08/1530, research and development project: Name: Objasnění úlohy proteinu HMGB1 v udržování genomové stability

Investor: Czech Science Foundation, Understanding of HMGB1 protein involvement in maintaining genome stability

• IAA500040801, research and development project: Name: Telomery a telomerázy: posun od molekulárně-biologického ke strukturně-biologickému přístupu.

Investor: Academy of Sciences of the Czech Republic, Telomeres and telomerase: transition from molecular to structural biology approach

• LC06035, research and development project: Name: Centrum biofyzikální chemie, bioelektrochemie a bioanalýzy. Nové nástroje pro genomiku, proteomiku a biomedicínu.

Investor: Ministry of Education, Youth and Sports of the CR, Centre of Biophysical Chemistry, Bioelectrochemistry and Bioanalysis. New Tools for Genomics, Proteomics and Biomedicine

• MSM0021622415, plan (intention): Name: Molekulární podstata buněčných a tkáňových regulací

Investor: Ministry of Education, Youth and Sports of the CR, Molecular basis of cell and tissue regulations