HLAVÁČEK, Antonín, Pavel BOUCHAL and Petr SKLÁDAL. Biotinylation of quantum dots for application in fluoroimmunoassays with biotin-avidin amplification. Microchimica Acta. Wien: Springer-Verlag, 2012, vol. 176, 3-4, p. 287-293. ISSN 0026-3672. Available from: https://dx.doi.org/10.1007/s00604-011-0729-6. |
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@article{964159, author = {Hlaváček, Antonín and Bouchal, Pavel and Skládal, Petr}, article_location = {Wien}, article_number = {3-4}, doi = {http://dx.doi.org/10.1007/s00604-011-0729-6}, keywords = {biotin; avidin; albumin; bioconjugation}, language = {eng}, issn = {0026-3672}, journal = {Microchimica Acta}, title = {Biotinylation of quantum dots for application in fluoroimmunoassays with biotin-avidin amplification}, volume = {176}, year = {2012} }
TY - JOUR ID - 964159 AU - Hlaváček, Antonín - Bouchal, Pavel - Skládal, Petr PY - 2012 TI - Biotinylation of quantum dots for application in fluoroimmunoassays with biotin-avidin amplification JF - Microchimica Acta VL - 176 IS - 3-4 SP - 287-293 EP - 287-293 PB - Springer-Verlag SN - 00263672 KW - biotin KW - avidin KW - albumin KW - bioconjugation N2 - A competitive microplate fluoroimmunoassay was developed for the determination of human serum albumin in urine. It is based on the use of biotinylated CdTe quantum dots (QDs) whose synthesis is optimised in terms of storage stability, purification, and signal-to-noise ratio. The bioconjugated QDs were characterised by gel chromatography and gel electrophoresis. Storage stability and quantum yield were investigated. The excitation/emission wavelengths are 360/620 nm. The immunoassay of human serum albumin in urine has a working range from 1.7 to 10 ug/mL, and the limit of detection is 1.0 ug/mL. ER -
HLAVÁČEK, Antonín, Pavel BOUCHAL and Petr SKLÁDAL. Biotinylation of quantum dots for application in fluoroimmunoassays with biotin-avidin amplification. \textit{Microchimica Acta}. Wien: Springer-Verlag, 2012, vol.~176, 3-4, p.~287-293. ISSN~0026-3672. Available from: https://dx.doi.org/10.1007/s00604-011-0729-6.
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