J
2012
Biotinylation of quantum dots for application in fluoroimmunoassays with biotin-avidin amplification
HLAVÁČEK, Antonín, Pavel BOUCHAL and Petr SKLÁDAL
Basic information
Original name
Biotinylation of quantum dots for application in fluoroimmunoassays with biotin-avidin amplification
Name in Czech
Biotinylace kvantových teček pro použití ve fluorescenčních imunostanoveních s biotin-avidinovým zesílením signálu
Authors
HLAVÁČEK, Antonín (203 Czech Republic, belonging to the institution),
Pavel BOUCHAL (203 Czech Republic, belonging to the institution) and
Petr SKLÁDAL (203 Czech Republic, guarantor, belonging to the institution)
Edition
Microchimica Acta, Wien, Springer-Verlag, 2012, 0026-3672
Other information
Type of outcome
Článek v odborném periodiku
Field of Study
10600 1.6 Biological sciences
Country of publisher
Austria
Confidentiality degree
není předmětem státního či obchodního tajemství
Impact factor
Impact factor: 3.434
RIV identification code
RIV/00216224:14310/12:00059178
Organization unit
Faculty of Science
Keywords (in Czech)
biotin; avidin; albumin; biokonjugace
Keywords in English
biotin; avidin; albumin; bioconjugation
Tags
International impact, Reviewed
V originále
A competitive microplate fluoroimmunoassay was developed for the determination of human serum albumin in urine. It is based on the use of biotinylated CdTe quantum dots (QDs) whose synthesis is optimised in terms of storage stability, purification, and signal-to-noise ratio. The bioconjugated QDs were characterised by gel chromatography and gel electrophoresis. Storage stability and quantum yield were investigated. The excitation/emission wavelengths are 360/620 nm. The immunoassay of human serum albumin in urine has a working range from 1.7 to 10 ug/mL, and the limit of detection is 1.0 ug/mL.
In Czech
Kvantové fluorescenční nanočástice byly modifikovány vrstvou redukovaného albuminu a dále derivatizovány biotinem. Tento biokonjugát posloužil v modelovém stanovení koncentrace albuminu v moči pomocí fluorescenčního imunostanovení.
Links
| LC06030, research and development project | | Name: Biomolekulární centrum | | Investor: Ministry of Education, Youth and Sports of the CR, Biomolecular centre |
|
| MSM0021622413, plan (intention) | | Name: Proteiny v metabolismu a při interakci organismů s prostředím | | Investor: Ministry of Education, Youth and Sports of the CR, Proteins in metabolism and interaction of organisms with the environment |
|
Displayed: 9/11/2024 11:36