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@article{964159,
author = {Hlaváček, Antonín and Bouchal, Pavel and Skládal, Petr},
article_location = {Wien},
article_number = {3-4},
doi = {http://dx.doi.org/10.1007/s00604-011-0729-6},
keywords = {biotin; avidin; albumin; bioconjugation},
language = {eng},
issn = {0026-3672},
journal = {Microchimica Acta},
title = {Biotinylation of quantum dots for application in fluoroimmunoassays with biotin-avidin amplification},
volume = {176},
year = {2012}
}
TY - JOUR
ID - 964159
AU - Hlaváček, Antonín - Bouchal, Pavel - Skládal, Petr
PY - 2012
TI - Biotinylation of quantum dots for application in fluoroimmunoassays with biotin-avidin amplification
JF - Microchimica Acta
VL - 176
IS - 3-4
SP - 287-293
EP - 287-293
PB - Springer-Verlag
SN - 00263672
KW - biotin
KW - avidin
KW - albumin
KW - bioconjugation
N2 - A competitive microplate fluoroimmunoassay was developed for the determination of human serum albumin in urine. It is based on the use of biotinylated CdTe quantum dots (QDs) whose synthesis is optimised in terms of storage stability, purification, and signal-to-noise ratio. The bioconjugated QDs were characterised by gel chromatography and gel electrophoresis. Storage stability and quantum yield were investigated. The excitation/emission wavelengths are 360/620 nm. The immunoassay of human serum albumin in urine has a working range from 1.7 to 10 ug/mL, and the limit of detection is 1.0 ug/mL.
ER -
HLAVÁČEK, Antonín, Pavel BOUCHAL and Petr SKLÁDAL. Biotinylation of quantum dots for application in fluoroimmunoassays with biotin-avidin amplification. \textit{Microchimica Acta}. Wien: Springer-Verlag, vol.~176, 3-4, p.~287-293. ISSN~0026-3672. doi:10.1007/s00604-011-0729-6. 2012.
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