The antibiotic resistance determinants in Staphylococcus aureus are localized mostly on mobile genetic elements, such as plasmids, transposons and SCCmec, and they can be horizontally transferred in bacterial populations. This contributes to rapid evolution of S. aureus strains and to increasing emergence of multiresistant strains of this important bacterial pathogen. The main aim of the study was to confirm and characterize the transfer of resistance plasmids by transduction between clinical isolates belonging to S. aureus USA300 clone. Two penicillinase plasmids and the tetracycline resistance plasmid pT181 were successfuly transduced from a set of donor strains into several recipient strains by transducing bacteriophages 53, 80alpha and JB. High frequencies of transduction were observed both in using propagated and induced phage lysates. The genotypes of transductants were confirmed by PCR assays, PFGE and restriction analysis of plasmid DNA. The quantification of plasmid molecules packaged in phage capsids was performed by real-time PCR. Moreover, recently isolated phage JB proved an outstanding transduction ability and has been chosen for further molecular analyses including whole-genome sequencing.