Enhanced osteoblast and osteoclast responses to a thin film
sputtered hydroxyapatite coating

J 2011

Enhanced osteoblast and osteoclast responses to a thin film sputtered hydroxyapatite coating

HAO, J., S. KURODA, K. OHYA, Sonia BARTÁKOVÁ, H. AOKI et. al.

Basic information

Original name

Enhanced osteoblast and osteoclast responses to a thin film sputtered hydroxyapatite coating

Authors

HAO, J. (392 Japan), S. KURODA (392 Japan), K. OHYA (392 Japan), Sonia BARTÁKOVÁ (203 Czech Republic, guarantor, belonging to the institution), H. AOKI (392 Japan) and Shohei KASUGAI (392 Japan)

Edition

JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE, Japan, 2011, 0957-4530

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30200 3.2 Clinical medicine

Country of publisher

Japan

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 2.316

RIV identification code

RIV/00216224:14110/11:00054875

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1007/s10856-011-4329-0

UT WoS

000291702500013

Keywords (in Czech)

implantát; povlakování; hydroxyapatite

Keywords in English

implant; coating; hydroxyapatite

Abstract

V originále

A sputtering technique followed by a low temperature hydrothermal treatment has been demonstrated to produce a dense-and-bioactive hydroxyapatite thin film coating. The purpose of the present study was to investigate osteoblast and osteoclast responses to the hydroxyapatite coated plates and titanium plates with similar roughness. Rat bone marrow stromal cells were cultured on these plates to induce osteoblasts. The cells showed a significantly enhanced proliferation on the hydroxyapatite surface, accompanied by increase of osteoblastic phenotypes. The co-cultured osteoclasts exhibited the significantly different cell number and morphology between the hydroxyapatite and the titanium surfaces. A series of osteoclast marker genes were more stimulated on the hydroxyapatite and thirty two percent of the hydroxyapatite surface area could be resorbed by osteoclasts. The thin film sputtered hydroxyapatite could provide a favorable surface for both osteoblast and osteoclast formation and their function, indicating its good osteoconductivity and biodegradability.

Links

1M0528, research and development project

Name: Stomatologické výzkumné centrum

Investor: Ministry of Education, Youth and Sports of the CR, Dental research centre

Citovat

HAO, J., S. KURODA, K. OHYA, Sonia BARTÁKOVÁ, H. AOKI and Shohei KASUGAI. Enhanced osteoblast and osteoclast responses to a thin film sputtered hydroxyapatite coating. JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE. Japan, 2011, vol. 22, No 6, p. 1489-1499. ISSN 0957-4530. Available from: https://dx.doi.org/10.1007/s10856-011-4329-0.

@article{965969,
   author = {Hao, J. and Kuroda, S. and Ohya, K. and Bartáková, Sonia and Aoki, H. and Kasugai, Shohei},
   article_location = {Japan},
   article_number = {6},
   doi = {http://dx.doi.org/10.1007/s10856-011-4329-0},
   keywords = {implant; coating; hydroxyapatite},
   language = {eng},
   issn = {0957-4530},
   journal = {JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE},
   title = {Enhanced osteoblast and osteoclast responses to a thin film sputtered hydroxyapatite coating},
   volume = {22},
   year = {2011}
}

TY  - JOUR
ID  - 965969
AU  - Hao, J. - Kuroda, S. - Ohya, K. - Bartáková, Sonia - Aoki, H. - Kasugai, Shohei
PY  - 2011
TI  - Enhanced osteoblast and osteoclast responses to a thin film sputtered hydroxyapatite coating
JF  - JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE
VL  - 22
IS  - 6
SP  - 1489-1499
EP  - 1489-1499
SN  - 09574530
KW  - implant
KW  - coating
KW  - hydroxyapatite
N2  - A sputtering technique followed by a low temperature hydrothermal treatment has been demonstrated to produce a dense-and-bioactive hydroxyapatite thin film coating. The purpose of the present study was to investigate osteoblast and osteoclast responses to the hydroxyapatite coated plates and titanium plates with similar roughness. Rat bone marrow stromal cells were cultured on these plates to induce osteoblasts. The cells showed a significantly enhanced proliferation on the hydroxyapatite surface, accompanied by increase of osteoblastic phenotypes. The co-cultured osteoclasts exhibited the significantly different cell number and morphology between the hydroxyapatite and the titanium surfaces. A series of osteoclast marker genes were more stimulated on the hydroxyapatite and thirty two percent of the hydroxyapatite surface area could be resorbed by osteoclasts. The thin film sputtered hydroxyapatite could provide a favorable surface for both osteoblast and osteoclast formation and their function, indicating its good osteoconductivity and biodegradability.
ER  -

HAO, J., S. KURODA, K. OHYA, Sonia BARTÁKOVÁ, H. AOKI and Shohei KASUGAI. Enhanced osteoblast and osteoclast responses to a thin film sputtered hydroxyapatite coating. \textit{JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE}. Japan, 2011, vol.~22, No~6, p.~1489-1499. ISSN~0957-4530. Available from: https://dx.doi.org/10.1007/s10856-011-4329-0.

Detailed Information on Publication Record