Bacteriocin genes among E. coli strains: the incidence depends on genotype and phenotype of E. coli strains

ŠMAJS, David, Barbora ŠTAUDOVÁ, Lenka MICENKOVÁ, Jan ŠMARDA, Martin VRBA, Alena ŠEVČÍKOVÁ, Zuzana VALIŠOVÁ and Vladana WOZNICOVÁ. Bacteriocin genes among E. coli strains: the incidence depends on genotype and phenotype of E. coli strains. In Acta Microbiologica et Immunologica Hungarica, vol. 58, Supplement : 16th International Congress of the Hungarian Society for Microbiology. 2011. ISSN 1217-8950.

Basic information

• Original name: Bacteriocin genes among E. coli strains: the incidence depends on genotype and phenotype of E. coli strains
• Authors: ŠMAJS, David (203 Czech Republic, guarantor, belonging to the institution), Barbora ŠTAUDOVÁ (203 Czech Republic, belonging to the institution), Lenka MICENKOVÁ (203 Czech Republic, belonging to the institution), Jan ŠMARDA (203 Czech Republic, belonging to the institution), Martin VRBA (203 Czech Republic), Alena ŠEVČÍKOVÁ (203 Czech Republic), Zuzana VALIŠOVÁ (203 Czech Republic) and Vladana WOZNICOVÁ (203 Czech Republic, belonging to the institution).
• Edition: Acta Microbiologica et Immunologica Hungarica, vol. 58, Supplement : 16th International Congress of the Hungarian Society for Microbiology, 2011.

Other information

• Original language: English
• Type of outcome: Conference abstract
• Field of Study: 10600 1.6 Biological sciences
• Country of publisher: Hungary
• Confidentiality degree: is not subject to a state or trade secret
• Impact factor: Impact factor: 0.787
• RIV identification code: RIV/00216224:14110/11:00050393
• Organization unit: Faculty of Medicine
• ISSN: 1217-8950
• Keywords in English: E. coli; bacteriocin; colicin; microcin; phylogroup

Abstract

E. coli strains (n = 1670) isolated during years 2007-2010 in the Czech Republic were collected from patients attending regional hospitals in Brno. All strains were tested using commercially available biochemical tests (ENTEROtest 16; Erba Lachema, Ltd., Brno, Czech Republic) for their biochemical activities, and with PCR for the presence to one out of four E. coli phylogroups including group A, B1, B2, and D. Moreover, the incidence of 29 bacteriocin-encoding determinants (i.e. colicin and microcin encoding genes) was tested in each individual E. coli strain. According to their biochemical activity, 1670 E. coli strains clustered into 6 groups containing 709, 50, 47, 744, 103, and 17 strains, respectively. The two largest clusters differed in the incidence of both phylogroups B2 and D and also in the incidence of bacteriocin determinants of mH47, mM, E1 and K. In addition, the incidence of colicinogenic strains was higher in the phylogroup A and B1 (p < 0.001) compared to combined B2 and D phylogroup strains. Strains of phylogroup B2 most frequently encoded microcin H47 and strains of phylogroup D microcin B17 and colicin Js. The incidence of individual bacteriocin encoding determinants is therefore predominantly associated with particular E. coli phylogroups and also with E. coli strains of specific biochemical activity.

Links

• GA310/07/0321, research and development project: Name: Komparativní genomové sekvencování patogenních treponem a transkriptomová analýza T. pallidum ssp. pallidum

Investor: Czech Science Foundation

• MSM0021622415, plan (intention): Name: Molekulární podstata buněčných a tkáňových regulací

Investor: Ministry of Education, Youth and Sports of the CR, Molecular basis of cell and tissue regulations

• NT11159, research and development project: Name: Mapování výskytu makrolidové rezistence původce syfilis v ČR a molekulární typizace jednotlivých syfilitických kmenů

Investor: Ministry of Health of the CR