In vitro modulation of intracellular receptor signaling and cytotoxicity induced by extracts of cyanobacteria, complex water blooms and their fractions.

ŠTĚPÁNKOVÁ, Tereza, L. AMBROŽOVÁ, Luděk BLÁHA, J.P. GIESY and Klára HILSCHEROVÁ. In vitro modulation of intracellular receptor signaling and cytotoxicity induced by extracts of cyanobacteria, complex water blooms and their fractions. Aquatic toxicology. AMSTERDAM, NETHERLANDS: ELSEVIER SCIENCE BV, 2011, vol. 105, 3-4, p. 497-507. ISSN 0166-445X. Available from: https://dx.doi.org/10.1016/j.aquatox.2011.08.002.

Basic information

• Original name: In vitro modulation of intracellular receptor signaling and cytotoxicity induced by extracts of cyanobacteria, complex water blooms and their fractions.
• Authors: ŠTĚPÁNKOVÁ, Tereza (203 Czech Republic, belonging to the institution), L. AMBROŽOVÁ (203 Czech Republic), Luděk BLÁHA (203 Czech Republic, belonging to the institution), J.P. GIESY (124 Canada) and Klára HILSCHEROVÁ (203 Czech Republic, guarantor, belonging to the institution).
• Edition: Aquatic toxicology, AMSTERDAM, NETHERLANDS, ELSEVIER SCIENCE BV, 2011, 0166-445X.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: 10511 Environmental sciences
• Country of publisher: Netherlands
• Confidentiality degree: is not subject to a state or trade secret
• Impact factor: Impact factor: 3.761
• RIV identification code: RIV/00216224:14310/11:00050448
• Organization unit: Faculty of Science
• Doi: http://dx.doi.org/10.1016/j.aquatox.2011.08.002
• UT WoS: 000298120600033
• Keywords in English: Cyanobacteria; Cell extract; Endocrine disruption; Estrogenicity; Microcystin; Planktothrix agardhii
• Tags: AKR, rivok

Abstract

The primary goal of this study was to determine if individual cyanobacterial species and mixtures of cyanobacteria collected from the environment contain compounds with the potential for interaction with signaling pathways of the aryl hydrocarbon receptor (AhR), androgen receptor (AR), estrogen receptor (ER), glucocorticoid receptor (GR) and retinoid acid receptor (RAR). Cytotoxicity and specific toxic potencies of products of freshwater cyanobacteria were determined by use of in vitro reporter gene trans-activation assays. Extracts of natural complex cyanobacterial biomasses exhibited greater and more frequent presence of compounds with specific modes of action, mainly estrogenic, and also greater cytotoxicity than extracts of single cyanobacterial species. The demonstrated estrogenic potency of the compounds present in complex cyanobacterial biomasses is of environmental relevance, and could potentially contribute to endocrine disruptive effects in aquatic ecosystems in case of great bloom densities.

Links

• ED0001/01/01, research and development project: Name: CETOCOEN
• GA524/08/0496, research and development project: Name: Mechanismy nádorové promoce metabolitů toxických sinic

Investor: Czech Science Foundation, Tumor promotional mechanisms of cyanobacterial metabolites

• MSM0021622412, plan (intention): Name: Interakce mezi chemickými látkami, prostředím a biologickými systémy a jejich důsledky na globální, regionální a lokální úrovni (INCHEMBIOL) (Acronym: INCHEMBIOL)

Investor: Ministry of Education, Youth and Sports of the CR, Interactions among the chemicals, environment and biological systems and their consequences on the global, regional and local scales (INCHEMBIOL)

• 2B08036, research and development project: Name: Nové molekulárně biologické a biochemické metody pro monitoring estrogenů a dalších chemických endokrinních disruptorů (Acronym: ENVISCREEN)

Investor: Ministry of Education, Youth and Sports of the CR, New molecular biology and biochemical methods to monitor estrogens and other endocrine disruptors in the environment of the C.R.