In vitro modulation of intracellular receptor signaling and cytotoxicity induced by extracts of cyanobacteria, complex water blooms and their fractions.

ŠTĚPÁNKOVÁ, Tereza, L. AMBROŽOVÁ, Luděk BLÁHA, J.P. GIESY and Klára HILSCHEROVÁ. In vitro modulation of intracellular receptor signaling and cytotoxicity induced by extracts of cyanobacteria, complex water blooms and their fractions. Aquatic toxicology. AMSTERDAM, NETHERLANDS: ELSEVIER SCIENCE BV, 2011, vol. 105, 3-4, p. 497-507. ISSN 0166-445X. Available from: https://dx.doi.org/10.1016/j.aquatox.2011.08.002.

Basic information

Original name

In vitro modulation of intracellular receptor signaling and cytotoxicity induced by extracts of cyanobacteria, complex water blooms and their fractions.

Authors

ŠTĚPÁNKOVÁ, Tereza (203 Czech Republic, belonging to the institution), L. AMBROŽOVÁ (203 Czech Republic), Luděk BLÁHA (203 Czech Republic, belonging to the institution), J.P. GIESY (124 Canada) and Klára HILSCHEROVÁ (203 Czech Republic, guarantor, belonging to the institution)

Edition

Aquatic toxicology, AMSTERDAM, NETHERLANDS, ELSEVIER SCIENCE BV, 2011, 0166-445X

---

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10511 Environmental sciences

Country of publisher

Netherlands

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 3.761

RIV identification code

RIV/00216224:14310/11:00050448

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1016/j.aquatox.2011.08.002

UT WoS

000298120600033

Keywords in English

Cyanobacteria; Cell extract; Endocrine disruption; Estrogenicity; Microcystin; Planktothrix agardhii

Tags

AKR, rivok

---

Abstract

V originále

The primary goal of this study was to determine if individual cyanobacterial species and mixtures of cyanobacteria collected from the environment contain compounds with the potential for interaction with signaling pathways of the aryl hydrocarbon receptor (AhR), androgen receptor (AR), estrogen receptor (ER), glucocorticoid receptor (GR) and retinoid acid receptor (RAR). Cytotoxicity and specific toxic potencies of products of freshwater cyanobacteria were determined by use of in vitro reporter gene trans-activation assays. Extracts of natural complex cyanobacterial biomasses exhibited greater and more frequent presence of compounds with specific modes of action, mainly estrogenic, and also greater cytotoxicity than extracts of single cyanobacterial species. The demonstrated estrogenic potency of the compounds present in complex cyanobacterial biomasses is of environmental relevance, and could potentially contribute to endocrine disruptive effects in aquatic ecosystems in case of great bloom densities.

---

Links

ED0001/01/01, research and development project	Name: CETOCOEN
GA524/08/0496, research and development project	Name: Mechanismy nádorové promoce metabolitů toxických sinic Investor: Czech Science Foundation, Tumor promotional mechanisms of cyanobacterial metabolites
MSM0021622412, plan (intention)	Name: Interakce mezi chemickými látkami, prostředím a biologickými systémy a jejich důsledky na globální, regionální a lokální úrovni (INCHEMBIOL) (Acronym: INCHEMBIOL) Investor: Ministry of Education, Youth and Sports of the CR, Interactions among the chemicals, environment and biological systems and their consequences on the global, regional and local scales (INCHEMBIOL)
2B08036, research and development project	Name: Nové molekulárně biologické a biochemické metody pro monitoring estrogenů a dalších chemických endokrinních disruptorů (Acronym: ENVISCREEN) Investor: Ministry of Education, Youth and Sports of the CR, New molecular biology and biochemical methods to monitor estrogens and other endocrine disruptors in the environment of the C.R.