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@article{971676,
author = {Krejčí, Pavel and Krakow, D. and Mekikian, P.B. and Wilcox, William R.},
article_location = {Rotterdam},
article_number = {3},
doi = {http://dx.doi.org/10.1203/pdr.0b013e318030d157},
keywords = {FACTOR RECEPTOR-3; THANATOPHORIC DYSPLASIA; CHONDROCYTE PROLIFERATION; NUCLEAR-LOCALIZATION; NIH-3T3 CELLS; BONE-GROWTH; FIBROBLAST; MOUSE; ACTIVATION;},
language = {eng},
issn = {0031-3998},
journal = {Pediatric Research},
title = {Fibroblast growth factors 1, 2, 17, and 19 are the predominant FGF ligandsexpressed in human fetal growth plate cartilage.},
volume = {61},
year = {2007}
}
TY - JOUR
ID - 971676
AU - Krejčí, Pavel - Krakow, D. - Mekikian, P.B. - Wilcox, William R.
PY - 2007
TI - Fibroblast growth factors 1, 2, 17, and 19 are the predominant FGF ligandsexpressed in human fetal growth plate cartilage.
JF - Pediatric Research
VL - 61
IS - 3
SP - 267-272
EP - 267-272
PB - Williams and Wilkins
SN - 00313998
KW - FACTOR RECEPTOR-3
KW - THANATOPHORIC DYSPLASIA
KW - CHONDROCYTE PROLIFERATION
KW - NUCLEAR-LOCALIZATION
KW - NIH-3T3 CELLS
KW - BONE-GROWTH
KW - FIBROBLAST
KW - MOUSE
KW - ACTIVATION;
N2 - Fibroblast growth factors (FGF) regulate bone growth, but their expression in human cartilage is unclear. Here, we determined the expression of entire FGF family in human fetal growth plate cartilage. Using reverse transcriptase PCR, the transcripts for FGFI, 2, 5. 8-14 16-19, and 21 were found. However, only FGFI, 2, 17, and 19 were detectable at the protein level. By immunohistochemistry, FGF17 and 19 were uniformly expressed within the growth plate. In contrast, FGF1 was found only in proliferating and hypertrophic chondrocytes whereas FGF2 localized predominantly to the resting and proliferating cartilage. In addition, only the 18 kD isoform of FGF2 was found in resting chondrocytes while proliferating chondrocytes also synthesized 22 kD and 24 kD FGF2, similar to in vitro cultivated chondrocytes. In cell growth experiments, FGFI, 2, and 17 but not FGF19 inhibited the proliferation of FGFR3-expressing rat chondrosarcoma chondrocytes (RCS) with relative potency FGF2 >> FGF1 = FGF17. We conclude that FGF1, 2, 17, and 19 are the predominant FGF ligands present in developing human cartilage that are, with the exception of FGF19, experimentally capable of inhibiting chondrocyte proliferation.
ER -
KREJČÍ, Pavel, D. KRAKOW, P.B. MEKIKIAN and William R. WILCOX. Fibroblast growth factors 1, 2, 17, and 19 are the predominant FGF ligandsexpressed in human fetal growth plate cartilage. \textit{Pediatric Research}. Rotterdam: Williams and Wilkins, 2007, vol.~61, No~3, p.~267-272. ISSN~0031-3998. Available from: https://dx.doi.org/10.1203/pdr.0b013e318030d157.
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