| KREJČÍ, Pavel, B. MASRI, V. FONTAINE, P.B. MEKIKIAN, M. WEIS, H. PRATS and W.R. WILCOX. Interaction of fibroblast growth factor and C-natriuretic peptide signaling inregulation of chondrocyte proliferation and extracellular matrix homeostasis. Journal of Cell Science. Cambridge: Company of Biologists Ltd., 2005, vol. 118, No 21, p. 5089-5100. ISSN 0021-9533. Available from: https://dx.doi.org/10.1242/jcs.02618. |
Other formats:
BibTeX
LaTeX
RIS
@article{971680,
author = {Krejčí, Pavel and Masri, B. and Fontaine, V. and Mekikian, P.B. and Weis, M. and Prats, H. and Wilcox, W.R.},
article_location = {Cambridge},
article_number = {21},
doi = {http://dx.doi.org/10.1242/jcs.02618},
keywords = {CYCLIC-GMP; ENDOCHONDRAL OSSIFICATION;INTERGLOBULAR DOMAIN; CARTILAGE AGGRECAN; PROTEIN-KINASES;},
language = {eng},
issn = {0021-9533},
journal = {Journal of Cell Science},
title = {Interaction of fibroblast growth factor and C-natriuretic peptide signaling inregulation of chondrocyte proliferation and extracellular matrix homeostasis.},
volume = {118},
year = {2005}
}
TY - JOUR
ID - 971680
AU - Krejčí, Pavel - Masri, B. - Fontaine, V. - Mekikian, P.B. - Weis, M. - Prats, H. - Wilcox, W.R.
PY - 2005
TI - Interaction of fibroblast growth factor and C-natriuretic peptide signaling inregulation of chondrocyte proliferation and extracellular matrix homeostasis.
JF - Journal of Cell Science
VL - 118
IS - 21
SP - 5089-5100
EP - 5089-5100
PB - Company of Biologists Ltd.
SN - 00219533
KW - CYCLIC-GMP
KW - ENDOCHONDRAL OSSIFICATION;INTERGLOBULAR DOMAIN
KW - CARTILAGE AGGRECAN
KW - PROTEIN-KINASES;
N2 - Overexpression of C-natriuretic peptide (CNP) in cartilage partially rescues achondroplasia in the mouse. Here, we studied the interaction of fibroblast growth factor (FGF) and CNP signaling in chondrocytes. CNP antagonized FGF2-induced growth arrest of rat chondrosarcoma (RCS) chondrocytes by inhibition of the Erk mitogen activated protein kinase pathway. This effect of CNP was protein kinase G-dependent and was mimicked by the cGMP analog pCPT-cGMP. FGF2-mediated activation of both MEK and Raf-1 but not Ras or FRS2 was abolished by CNP demonstrating that CNP blocks the Erk pathway at the level of Raf-1. CNP also counteracted the FGF2-mediated degradation of RCS extracellular matrix. CNP partially antagonized FGF2-induced expression, release and activation of several matrix-remodeling molecules including matrix metalloproteinase 2 (MMP2), MMP3, MMP9, MMP10 and MMP13. In addition, CNP compensated for FGF2-mediated matrix loss by upregulation of matrix production independent of its interference with FGF signaling. We conclude that CNP utilizes both direct and indirect ways to counteract the effects of FGF signaling in a chondrocyte environment.
ER -
KREJČÍ, Pavel, B. MASRI, V. FONTAINE, P.B. MEKIKIAN, M. WEIS, H. PRATS and W.R. WILCOX. Interaction of fibroblast growth factor and C-natriuretic peptide signaling inregulation of chondrocyte proliferation and extracellular matrix homeostasis. \textit{Journal of Cell Science}. Cambridge: Company of Biologists Ltd., 2005, vol.~118, No~21, p.~5089-5100. ISSN~0021-9533. Available from: https://dx.doi.org/10.1242/jcs.02618.
|
