Modulation of death receptor-mediated apoptosis in differentiating human myeloid leukemia HL-60 cells

VONDRÁČEK, Jan, M.A. SHEARD, Pavel KREJČÍ, Kateřina MINKSOVÁ, Jiřina HOFMANOVÁ a Alois KOZUBÍK. Modulation of death receptor-mediated apoptosis in differentiating human myeloid leukemia HL-60 cells. Online. Journal of Leukocyte Biology. New York: Society for Leukocyte Biology, 2001, roč. 69, č. 5, s. 794-802. ISSN 0741-5400. [citováno 2024-04-23]

Základní údaje

• Originální název: Modulation of death receptor-mediated apoptosis in differentiating human myeloid leukemia HL-60 cells
• Autoři: VONDRÁČEK, Jan, M.A. SHEARD, Pavel KREJČÍ, Kateřina MINKSOVÁ, Jiřina HOFMANOVÁ a Alois KOZUBÍK
• Vydání: Journal of Leukocyte Biology, New York, Society for Leukocyte Biology, 2001, 0741-5400.

Další údaje

• Originální jazyk: angličtina
• Typ výsledku: Článek v odborném periodiku
• Obor: 30105 Physiology
• Stát vydavatele: Spojené státy
• Utajení: není předmětem státního či obchodního tajemství
• Impakt faktor: Impact factor: 4.516
• Organizační jednotka: Přírodovědecká fakulta
• UT WoS: 000168806800016
• Klíčová slova anglicky: all-trans retinoic acid; dimethyl sulfoxide; Fas/CD95/APO-1; TNF-alpha; Bcl-2; FAP-1

Anotace

Differentiating myeloid cells may become resistant to various apoptotic stimuli. In the present study, dimethyl sulfoxide (DMSO) and all-trans retinoic acid (ATRA) were found to modulate the sensitivity of HL-60 cells to death receptor-mediated apoptosis in a time-dependent manner. During the early stages of differentiation, DMSO treatment increased the response of HL-60 cells to tumor necrosis factor cw; (TNF-alpha), but enhanced responsiveness was lost during later differentiation stages. In contrast, ATRA treatment induced resistance to TNF-alpha -induced apoptosis, HL-60 cells were resistant to Fas-mediated apoptosis but were sensitized by culturing in serum-free conditions. Similar to its effect on TNF-cr sensitivity, DMSO pretreatment augmented the response to Fas-mediated signaling, which coincided with increased expression of Fas on DMSO-pretreated cells. However, during the later stages of DMSO-induced differentiation, sensitivity to anti-Fas antibody-induced apoptosis declined significantly, although Fas expression was still elevated, The reduced sensitivity to anti-Fas treatment partially correlated with increased Fas-associated phosphatase-l mRNA expression, Thus, regardless of either Fas up-regulation or potentiation of TNF-alpha -mediated apoptosis during early DMSO-induced differentiation, a slow increase in resistance to apoptosis mediated through these death receptors occurs during DMSO-induced differentiation, which contrasts with the rapid induction of resistance following treatment with ATRA.