DOKLÁDAL, Ladislav, Michal OBOŘIL, Karel STEJSKAL, Zbyněk ZDRÁHAL, Nikola PTÁČKOVÁ, Radka CHALOUPKOVÁ, Jiří DAMBORSKÝ, Tomáš KAŠPAROVSKÝ, Jeandroz SYLVAIN, Markéta ŽĎÁRSKÁ and Jan LOCHMAN. Physiological and proteomic approaches to evaluate the role of sterol binding in elicitin-induced resistance. Journal of Experimental Botany. Oxford: OXFORD UNIV PRESS, 2012, vol. 63, No 5, p. 2203-2215. ISSN 0022-0957. doi:10.1093/jxb/err427.
Other formats:   BibTeX LaTeX RIS
Basic information
Original name Physiological and proteomic approaches to evaluate the role of sterol binding in elicitin-induced resistance
Authors DOKLÁDAL, Ladislav (203 Czech Republic, belonging to the institution), Michal OBOŘIL (203 Czech Republic, belonging to the institution), Karel STEJSKAL (203 Czech Republic, belonging to the institution), Zbyněk ZDRÁHAL (203 Czech Republic, belonging to the institution), Nikola PTÁČKOVÁ (203 Czech Republic, belonging to the institution), Radka CHALOUPKOVÁ (203 Czech Republic, belonging to the institution), Jiří DAMBORSKÝ (203 Czech Republic, belonging to the institution), Tomáš KAŠPAROVSKÝ (203 Czech Republic, belonging to the institution), Jeandroz SYLVAIN (250 France), Markéta ŽĎÁRSKÁ (203 Czech Republic, belonging to the institution) and Jan LOCHMAN (203 Czech Republic, guarantor, belonging to the institution).
Edition Journal of Experimental Botany, Oxford, OXFORD UNIV PRESS, 2012, 0022-0957.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10600 1.6 Biological sciences
Country of publisher United Kingdom of Great Britain and Northern Ireland
Confidentiality degree is not subject to a state or trade secret
Impact factor Impact factor: 5.242
RIV identification code RIV/00216224:14310/12:00057274
Organization unit Faculty of Science
Doi http://dx.doi.org/10.1093/jxb/err427
UT WoS 000301297800031
Keywords in English Elicitins; intercellular proteome; omega-loop conformation; sterol binding; resistance induction
Tags AKR, podil, rivok
Changed by Changed by: prof. Mgr. Tomáš Kašparovský, Ph.D., učo 10078. Changed: 8. 12. 2015 09:30.
Abstract
Cryptogein is a proteinaceous elicitor secreted by Phytophthora cryptogea that can induce resistance to P. parasitica in tobacco plants. On the basis of previous computer modelling experiments, by site-directed mutagenesis a series of cryptogein variants was prepared with altered abilities to bind sterols, phospholipids or both. The sterol binding and phospholipid transfer activities corresponded well with the previously reported structural data. Induction of the synthesis of reactive oxygen species (ROS) in tobacco cells in suspension and proteomic analysis of intercellular fluid changes in tobacco leaves triggered by these mutant proteins were not proportional to their ability to bind or transfer sterols and phospholipids. However, changes in the intercellular proteome corresponded to transcription levels of defence genes and resistance to P. parasitica and structureprediction of mutants did not reveal any significant changes in protein structure. These results suggest, contrary to previous proposals, that the sterol-binding ability of cryptogein and its mutants, and the associated conformational change in the v-loop, might not be principal factors in either ROS production or resistance induction. Nevertheless, the results support the importance of the v-loop for the interaction of the protein with the high affinity binding site on the plasma membrane.
Links
GAP501/11/1003, research and development projectName: Interakce mutantních forem cryptogeinu s membránovým vazebným místem: charakterizace pomocí biosensorů
Investor: Czech Science Foundation
GP203/09/P248, research and development projectName: Mechanizmus interakce ergosterolu s rostlinnou buňkou
Investor: Czech Science Foundation, Mechanism of ergosterol - plant cell interaction
MSM0021622413, plan (intention)Name: Proteiny v metabolismu a při interakci organismů s prostředím
Investor: Ministry of Education, Youth and Sports of the CR, Proteins in metabolism and interaction of organisms with the environment
MSM0021622415, plan (intention)Name: Molekulární podstata buněčných a tkáňových regulací
Investor: Ministry of Education, Youth and Sports of the CR, Molecular basis of cell and tissue regulations
PrintDisplayed: 20. 5. 2022 22:10